摘要
目的 建立标准肽图分析法 ,用于rhIL 11的质量控制。方法 应用AllianceHPLC系统及其温控自动进样器探索最佳胰蛋白酶切和色谱条件。结果 连续 3批rhIL 11样品的RP HPLC图谱完全一致 ,与rhIL 11对照品比较 ,有 2 0个峰与对照品吻合 ,但第 6峰的峰高和峰面积均明显较小 ,且在第 9和第 10峰之间多一个峰 ,说明rhIL 11样品蛋白质结构与对照品比较存在细小差别。这种差别经多肽片段分离和氨基酸序列测定证明是由于样品N端多 2个氨基酸引起的。结论 本法精确度高、重复性好、自动化程度高 ,可用于rhIL 11的质量控制。
AIM To establish a standard method of peptide mapping analysis for quality control of rhIL 11. METHODS To find out the best tryptic digestion and chromatogram conditions by using Alliance HPLC system and automatic temperature controlled injector. RESULTS Peptide mappings of three continuous batches of samples rhIL 11 are completely identical. Comparing with that of reference rhIL 11, 20 of the 21 peaks are all the same, but the area and height of the sixth peak are smaller than those of the reference product. Moreover, there is one extra peak between the ninth and tenth peak. It is stated that there are minor differences between the protein structure of the samples and that of the reference. The sixth and extra peaks of digested mixture of samples were separated and collected. Determination of amino acid sequence of two collected fragments indicates that there are two extra amino acids at the N terminal of the sample, which leads to the detail differences. CONCLUSION The method has high degree of accuracy, automation and fine duplication, which can be applied for quality control of rhIL 11.
出处
《药学学报》
CAS
CSCD
北大核心
2000年第5期378-380,共3页
Acta Pharmaceutica Sinica
基金
科技部国家重点科技项目!(攻关 )计划 ( 96 90 1 0 6 0 41)
关键词
重组人白细胞介素-11
肽图
胰蛋白酶切
recombinant human Interleukin 11
peptide mapping
alliance HPLC system
tryptic digestion
