摘要
使用In-Fusion试剂盒构建表达载体pCUB-Zein::ferritin-35s::bar,以玉米自交系齐319茎尖分生组织为受体,采用农杆菌介导法,将玉米胚乳特异启动子基因15 kDβ-Zein驱动的大豆铁蛋白ferritin基因转入玉米,共筛选出272株除草剂(草铵膦)抗性植株,其中108株PCR检测呈阳性,转化率达3.6%,初步判断Zein::ferritin基因已转入玉米基因组中。
The plant expression vector pCUB-Zein：：ferritin-35s：：bar was constructed by In-Fusion kit.The soybean ferritin gene was transferred into the shoot apical point of maize inbred line of maize inbred line Qi319 by Agrobacterium tumefacien mediated transformation.The soybean ferritin gene was expressed under the control of a maize seed storage protein prolamin promoter 15kD β-Zein.272 transgenic plants were obtained after select-ing with glufosinate and 108 of them were confirmed positive by PCR amplification.The frequency of transfor-mation reached about 3.6%.This study showed that the soybean ferritin gene was integrated into maize genome.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2012年第2期263-268,共6页
Journal of Anhui Agricultural University
基金
国际合作项目HarvestPlus China(HPC#8235)资助
