摘要
目的观察曲马多预先给药对急性心肌缺血大鼠心肌组织缺血区和非缺血区P物质(SP)表达的影响,探讨痛觉干预在缺血心肌保护中的作用。方法健康成年雄性SD大鼠18只,体重270-300 g,随机分为3组(各n=6):假手术组(S组)、单纯冠状动脉结扎组(I组)和曲马多干预冠状动脉结扎组(T组)。S组大鼠开胸后在冠状动脉左前降支下穿线不结扎;I组大鼠开胸后结扎冠状动脉左前降支;T组大鼠经尾静脉注射曲马多12.5 mg/kg,15 min后结扎冠状动脉左前降支。各组在手术后计时3 h。采用免疫组化、酶免疫试验和反转录-聚合酶链反应从蛋白和基因水平观察各组大鼠缺血区和非缺血区心肌SP的表达。结果 I组大鼠缺血区与非缺血区心肌SP和SP mRNA的水平均较S组升高(P<0.05),T组低于I组(P<0.05),但仍高于S组(P<0.05)。结论曲马多预先给药可降低急性心肌缺血大鼠心肌组织SP的表达,提示曲马多痛觉干预可能参与缺血心肌的保护。
Objective To investigate the effects of tramadol pretreatment on the expression of substance P（SP） in ischemia and non-ischemia myocardium following the acute myocardial ischemia.Methods Eighteen adult male Sprague-Dawley rats weighing 270-300 g were randomly divided into 3 groups（n=6 in each group）：sham group,ischemia group and tramadol group.The left anterior descending branch of coronary artery was occluded for 3 h in ischemia group and tramadol group.In tramadol group,tramadol 12.5 mg/kg was additionally injected through caudal vein 15 min before coronary artery occlusion（CAO）.The hearts were removed at the end of 3 h for determining the SP protein expression by immunohistochemistry and enzyme immunometric assay and the expression of SP mRNA by RT-PCR in ischemia and non-ischemia myocardium.Results Compared with sham group,SP and SP mRNA levels were increased significantly in ischemia group and tramadol group in myocardium of ischemic and non-ischemic areas（P0.05）,but the levels were significantly higher in ischemia group than those in tramadol group（P0.05）.Conclusion Tramadol pretreatment can significantly inhibit the SP expression in myocardium evoked by CAO,which suggests that tramadol pretreatment might take part in the cardioprotection against acute myocardial ischemia.
出处
《山西医科大学学报》
CAS
2012年第3期167-170,237,共5页
Journal of Shanxi Medical University
基金
国家自然科学基金资助项目(30471656)
关键词
曲马多
P物质
心肌缺血
tramadol
substance P
myocardial ischemia
