摘要
以表面氨基功能化纳米磁性复合微球为载体,戊二醛为交联剂,对纤维素酶的固定化条件进行研究。先通过单因素试验确定了温度、时间、pH值、交联剂戊二醛浓度和酶浓度等对固定化酶活性的影响,进而根据中心因子复合原则设计pH值、温度和酶浓度3个因素响应面试验。对试验结果进行二次回归分析,得到了响应面模型,预测最优固定化条件:温度为21.1℃,pH为4.2,酶浓度为0.076,该优化条件下,固定化酶活有所提高,能较好地与响应面模型预测值相吻合。
Cellulase was immobilized on the amino-silane modified magnetic nanoparticles by using glutaradehyde as the cross-linker.Firstly,the immobilization conditions were optimized through single-factor test,such as,temperature,time,pH,glutaraldehyde concentration and cellulase concentration.20 experiments were performed according to central composite design（CCD） of three factors（temperature,pH value and glutaraldehyde concentration）.Response surface model（RSM） was obtained by quadratic regression analysis of experimental data of CCD.The results showed that the optimum condition of cellulase immobilization was 21.1 ℃,pH 4.2 and 0.076（v/v） cellulose concentration.Under optimum condition,the actual relative activity of the immobilized cellulase activity was increased,which could accord well with the predicted value.
出处
《可再生能源》
CAS
北大核心
2012年第4期80-84,共5页
Renewable Energy Resources
基金
国家科技支撑计划(2011BAD22B01)
国家高技术研究发展计划("863"计划)(2007AA05Z406)
中科院知识创新工程重要方向项目(KSCX2-YW-G-075-09
KGCX2-YW-335和KSCX-YW-11-A3)
中国科学院可再生能源与天然气水合物重点实验室开放基金(0907K6)
