摘要
目的 主要探讨一氧化氮（ＮＯ） 和诱导型一氧化氮合酶（ｉＮＯＳ）在内毒素（ＬＰＳ） 诱导的大鼠急性肺损伤（ＡＬＩ）的作用机制及大黄对其影响。方法 在雄性Ｗｉｓｔａｒ 大鼠利用舌下静脉注射ＬＰＳ复制ＡＬＩ动物模型，动物分为４ 组：ＬＰＳ组、对照组、大黄治疗组、地塞米松组。观察大体标本，组织病理以及生物学标志：肺湿／干重比、肺泡灌洗液中性粒细胞比、蛋白含量、肺血管通透性和肺泡通透性指数。同时测定血浆ＮＯ和肺组织匀浆ｉＮＯＳ活性。结果 ＬＰＳ组与对照组相比，肺组织病理显示肺间质及肺泡明显损伤和细胞浸润。其生物学标志均显著升高（Ｐ＜０－０１），ＮＯ 和ｉＮＯＳ也显著升高（ Ｐ＜０－０１） ，地塞米松和大黄干预治疗组则表现组织病理明显减轻。上述肺损伤生物标记物及ＮＯ、ｉＮＯＳ也相应下降（ Ｐ＜ ０－０５） 。结论 在ＬＰＳ诱导的ＡＬＩ大鼠动物模型中证明ＮＯ和ｉＮＯＳ在ＡＬＩ的发病过程中起到较为关键性的作用，用地塞米松、大黄进行干预治疗，可使损伤减轻相应地也使ＮＯ、ｉＮＯＳ浓度下降，表明这２ 种药物对ＬＰＳ诱导的ＡＬＩ具有保护作用，其机制可能是通过抑制ＮＯ和ｉＮＯＳ活性实现。
Objective To approach mainly the actions of nitric oxide(NO)and inducible nitric oxide synthase (iNOS) on endotoxin (lipopolysaccharidePaO 2<6 7kPa,LPS)induced rat acute lung injury (ALI) and the affeciton of rhubarb on them.Methods LPS was injected into the subingual vein of male Wistar rats to prepare ALI animal models.The rats were divided into the 4 groups:LPS group,control group,rhubarb group and dexamethasone group.Macroscopic and histopathological examinations were performed and biological indexes were measured for the lung specimens.The indexes included lung wet weight/dry weight,the rats of neutrophils and protein content in the pulmonary alveolar lavage fluid,pulmonary vascular permeability and pulmonary alveolar permeability index.In the mean time,the activities of serum NO and lung tissue homogenate iNOS were measured.Results In the comparison of the lung histopathological examination between the LPS group and control group,the injury and cellular infiltration in the pulmonary stroma and alveoli were more prominent in the LPS group than in the control group.Lung wet weight/dry weight,rat of neutrophils,protein content,pulmonary alveolar permeability index,pulmonary vascular permeability were significantly increased ( P <0 01);NO and iNOS were also markedly elevated ( P <0 01).In the groups with dexamethasone and rhubarb intering treatment,the histopathological changes were singnificantly milder,and the above biological indexes of lung injury and the contents of NO and iNOS were correspondingly decreased ( P <0 05).Conclusions From the LPS induced ALI rat models,it is demonstrated that NO and iNOS play important roles in the onset of ALI:dexamethasone and rhubarb interfering treatment can ameliorate,the lung injury and decrease the concentrations of NO and iNOS,showing that these 2 agents have protective effect on LPS induced ALI and their therapeutic mecharnism is possibly through inhibiting the activities of NO and iNOS. [
出处
《中国急救医学》
CAS
CSCD
北大核心
2000年第2期65-68,共4页
Chinese Journal of Critical Care Medicine
关键词
一氧化氮
INOS
肺损伤
内毒素
大黄
Nitric oxide(NO)
lnducible nitric oxide synthase (iNOS)
Acute lung injury (ALI)
Endotoxin
Rhubarb
Dexamethasone
