摘要
目的分析克雷伯菌属的耐药性和耐药基因载体-可移动原件的携带情况。方法收集宁波大学医学院附属医院及宁波市医疗中心李惠利医院2009年8月-2011年3月住院患者送检标本分离出20株克雷伯菌属,经鉴定确认2株为植生克雷伯菌,18株为肺炎克雷伯菌;采用K-B纸片扩散法对其进行13种抗菌药物敏感性判断;PCR法检测13种可移动遗传元件,PCR阳性产物采用PCR直接全自动荧光法测序。结果 20株克雷伯菌属均表现对β-内酰胺类、氨基糖苷类、喹诺酮类抗菌药物多药耐药性,植生克雷伯菌对亚胺培南和美罗培南敏感;20株克雷伯菌属的可移动遗传元件检出率分别为:traA10.0%、trbC70.0%、intⅠ1 100.0%、tnpU80.0%、tnp513 100.0%、IS26 100.0%、IS903 95.0%、ISKpn6 45.0%、ISEcp1 90.0%。结论 20株克雷伯菌属多药耐药的表型可能与携带多种可移动遗传元件相关。
OBJECTIVE To investigate the drug resistance of Klebsiella spp and their mobile genetic elements. METHODS A total of 20 strains of Klebsiella isolated from patients hospitalized in the Affiliated Hospital of Medical College of Ningbo University and Ningbo Treatment Center Lihuili Hospital ：from Aug. 2009 to Mar. 2011 were collected. The identification confirmed that two strains were K. planticola, and 18 stains were Kleb siella pneumonia. The drug susceptibility to 13 antibiotics was determined by KB test. Thirteen mobile genetic elements were detected by PCR and verified by automatic fluorescence gene sequencing for PCR positive products. RRESULTS Totally 20 Klebsiella isolates were multidrugresistant to β1actams, aminoglycosides, carbapenem and quinolone antibacterials. K. plant：cola isolates were susceptibility to imipenem and meropenem. The detection rates of mobile genetic elements were as the followsing, traA 10. 0%, trbC 70. 0%, int I 1 100. 0%, tnpU 80.0%, tnpS13 100.0%, IS26 100. 0%, IS903 95. 0%, ISKpn6 45. 0%, ISEcpl 90. 0%, respectively. CONCLUSION The multidrug resisl：ance of Klebsiella spp may be correlated to carrying of multiple mobile genetic elements.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2012年第7期1325-1328,共4页
Chinese Journal of Nosocomiology
