摘要
将质粒pcDNA3.1-VEGF双酶切后亚克隆至逆转录病毒载体pLXSN,并用酶切及测序等手段进行鉴定.而后利用脂质体转染包装细胞pA317,并检测病毒滴度.结果表明:成功构建了VEGF基因的重组逆转录病毒表达载体.
According to compatible restriction sites, we digest the plasmid pcDNA3.1 -VEGF and pLXSN and connect them together,then perform enzyme cutting and sequence analysis to identify the connection. Lipofeetamine was Used to me- diate recombinant plasmid pLXSN - VEGF transfection of pA317 packaging cells, collect virus - containing medium from packaging cells, infect NIH3T3 cells to determine viral titer. The results indicate that retroviral vector pLXSN- VEGF was successfully established.
出处
《周口师范学院学报》
CAS
2012年第2期79-80,139,共3页
Journal of Zhoukou Normal University
