摘要
目的 通过体外培养的猪眼小梁细胞,观察白细胞介素1-α（interleukin-1α,IL-1α）对体外培养的猪眼小梁细胞P38通路的影响,以期发现青光眼发病的病理生理机制.方法 取猪眼小梁组织进行小梁细胞体外培养,取传3～5代的猪眼小梁细胞,分对照组和IL-1α组,采用免疫组织化学染色检测培养的猪眼小梁细胞P38的表达情况;Western blotting技术检测小梁细胞P38通路磷酸化水平的变化.组间计数资料的比较,采用χ2检验或Fisher精确概率计算法;两组数据中的蛋白条带的光强度值,采用t检验统计学方法.结果 体外培养的猪眼小梁细胞大约2周左右开始从组织块表面爬出.未经IL-1α刺激的猪眼小梁细胞行免疫组织化学染色方法表达P38的量很少,而经IL-1刺激后的小梁细胞P38表达量明显增加（P〈0.05）.经Il-1刺激后的猪眼小梁细胞行Western blotting技术检测,目的 蛋白的光强度值明显高于对照组（P〈0.05）.各组数据之间的差异均有统计学意义.结论 外源性IL-1α对体外培养的猪眼小梁细胞P38通路产生影响,使p38的表达量增加.因此,外源性氧化应激可能影响小梁细胞P38通路,p38进一步调控小梁细胞内的微环境变化,影响眼压变化.
Objective To detect glaucoma pathogenesis,ohservation of interleukin 1-α（IL-1α） on the in vitro development of porcihe trahecular cells P38 pathway influences,through in vitro euhure of porcine trabecular cells. Methods The research is experimental. Porcine traheeular tissue was used and trabeeular cells was cultured in vitro,and 3 -5 generation of porcine trabeeular cells was selected, which were divided into control group and IL-1α grouts, inmmnohistoehemical was used for detecting staining of euhured porcine Irabecular Cell expression of P38 ; Western blotting technique for the detection of trabecular meshwork cells P38 pathway phosphorylalion level changes. Between group eomparison of count data,test or Fisher exact prohahility calculation method. Two sets of data in the protein bands of the light intensity value,test of statistical method was used. Results In vitro culture of porcine trabecular eells for about 2 weeks started from the tissue surface climb out. The expression of P38 in small quantities,when porcine trabeeular meshwork cell line by inmmnohistochemical staining method without IL-1α slimulates,but Ihe expression of of P38 in the Iraheeular meshwork increased significantly （P 〈 0. 05）. The target prote was detected by western blotting technique,when the porcine traheeular cells after stimulation of IL-1α,light intensity was significantly higher than that of control group（ P 〈0.05）. The data of eaeh group had significant differences. Conclusion Effect of exogenous IL-1α on cultured pored he Iraheular cells P38 palhway produces an effect,so can increase expression of p38. Therefore,exogenous oxidative stress may affect the Irabecular meshwork cells P38 pathway,p38 further regulate haheeular meshwork ceils within microfluidie environment change,and influence Ihe intraocular pressure.
出处
《潍坊医学院学报》
2012年第1期56-58,共3页
Acta Academiae Medicinae Weifang
