摘要
[目的]对重组融合蛋白的最佳诱导表达条件进行研究。[方法]为提高IMPACT-CN系统的pTYB11载体重组融合蛋白的表达量,针对其表达所需适宜的诱导时机、诱导剂量、诱导时间、诱导温度、氨苄青霉素浓度、摇瓶装液量等培养条件,采用不同技术指标进行了平行发酵试验。[结果]表达产物的SDS-PAGE及Western-Blotting分析表明重组融合蛋白具有良好免疫原性。[结论]为以后的蛋白纯化诊断、用蛋白质生产诊断试剂盒及其临床应用奠定了基础。
[Objective] To study the best induced conditions for expression of recombinant fusion protein.[Method] The best initial OD600,induction amount,induction duration,induction temperature,ampicillin concentration,liquid volume and other culture conditions were explored by using parallel fermentation experiments with different technical indicators to improve the expression level of the recombinant fusion protein of IMPACT-CN pTYB11 vector.[Result] Expression of SDS-PAGE and Western-Blotting analysis showed that the recombinant fusion protein has a high immunogenicity.[Conclusion] This study laid the foundation for further protein purification in diagnosis,production of diagnostic kits with protein and clinical application.
出处
《安徽农业科学》
CAS
2012年第10期5743-5745,5830,共4页
Journal of Anhui Agricultural Sciences
基金
滨州职业学院科研项目(07XYA04)
关键词
克隆
表达
融合蛋白
Cloning
Expression
Fusion protein
