摘要
目的:观察柴胡疏肝散对慢性胰腺炎大鼠肝脏胰岛素抵抗和核因子-κB活性的干预作用。方法:Wistar大鼠制备慢性胰腺炎模型后,每日给予柴胡疏肝散1.4 g/kg和2.8 g/kg体重灌胃4周,然后进行葡萄糖耐量和胰岛素耐量实验,分离肝细胞进行葡萄糖释放实验,检测肝脏组织核因子-κB活性变化。结果:慢性胰腺炎大鼠的糖耐量异常,胰岛素敏感性降低,胰岛素对离体肝细胞葡萄糖释放的抑制率减弱,肝脏核因子-κB的活性显著增加(P<0.05);柴胡疏肝散干预能够显著改善上述变化(P<0.05)。结论:柴胡疏肝散能够抑制肝脏核因子-κB的过度活化,进而改善慢性胰腺炎引发的肝脏胰岛素抵抗。
Objective： To investigate the effects of chaihushugansan on hepatic insulin resistance and nuclear factor（NF）-κB activity in chronic pancreatitis（CP） rat.Methods： CP was induced in Wistar rats and chaihushugansan was administered by oral gavage at the doses 1.4 g/（kg.day） and 2.8 g/（kg.day） for 4 weeks respectively.Then glucose tolerance tests and insulin tolerance tests were performed.Hepatocytes were isolated for glucose release experiments.The NF-κB activity in liver was determinated by immunohistochemical method.Results： Induction of CP caused abnormal glucose tolerance,reduced insulin sensibility in rats and attenuated the capacity of insulin to suppress glucose release in isolated hepatocytes.Hepatic NF-κB activites were significantly increased in CP rats（P0.05）.In contrast,administration of chaihushugansan significantly（P0.05） attenuated the aforementioned changes.Conclusion： Treatment with chaihushugansan prevents hepatic insulin resistance caused by CP and has the effect in association with attenuation of increased hepatic NF-κB activites.
出处
《天津医科大学学报》
2012年第1期31-33,36,共4页
Journal of Tianjin Medical University
