摘要
目的探讨荧光原位杂交(FISH)技术用于早期难免流产绒毛间期细胞染色体非整倍体异常的临床应用价值。方法采用FISH技术对20例我院住院难免流产病人的流产绒毛进行5条染色体(21、13、18、X和Y)的快速检测。结果被检测的20例样本中,用FISH检测,均获得诊断结果,检测成功率为100%,FISH检测结果中有8例异常,异常率为40%,其中XO是难免流产中胚胎中最多的异常。结论应用FISH技术检测未培养绒毛间期细胞染色体数目异常,具有快速,简便,使用样本量少等优势,具有一定的临床应用价值。
Objective： To evaluate the value of applying fluorescent in situ hybridization in diagnosis on chromosomal aneuploidies of villous cells in inevitable abortion.Methods： Chorionic villi from 20 pregnant women with inevitable abortion in our hospital were selected.Five chromosomes（21、13、18、X和Y）were detected with FISH technology.Results： Of all the samples,20 samples were successfully tested by FISH,8 cases were abnormal,the abnormal rate was 40%,XO was the most common abnormality in nevitable abortion.Conclusion： FISH technology used in diagnosis on chromosomal abnormalities in uncultured villous cells showed highly efficient,low cost,and small amounts of samples needed,and holds an important clinical value.
出处
《中国优生与遗传杂志》
2012年第4期38-39,共2页
Chinese Journal of Birth Health & Heredity
关键词
绒毛细胞
荧光原位杂交
非整倍体
Villous Cells
Fluorescence in situ hybridization
Aneuploidy
