摘要
本实验研究了转输615系小鼠脾LAK细胞和LAK—88/DC细胞系对615小鼠白血病(L615)的免疫防治效应,以及继承性化学免疫治疗作用。实验结果表明,攻击前二十一天转输LAK细胞或LAK—88/DC细胞系,并每日腹腔注射培养上清液一周,可以诱导正常受鼠产生对L615明显的免疫预防效应,分别使实验组80%的小鼠长期存活;单独注射培养上清液也有效,可使60%的小鼠长期存活。在攻击后五天,用环磷酰胺(CY:180mg/kg)进行化疗并转输LAK细胞或LAK—88/DC细胞系,则能延长晚期L615病鼠的平均存活时间,并可使实验组25%～28.6%的小鼠长期存活。
In the present experiment, we investigated the im munoprophylactic and immunotherapeutic effects of transferring spleen LAK cells or ' LAK-88/DC ' cells from 615 inbred mice against mouse leukemia. The results showed that transferring LAK cells or 'LAK-88/DC' cells and injecting conditional medium for a week 21 days before L615 inoculation induced significant prophylactic effects, which cured 80% of the L615-sufferring mice. Injecting conditional medium alone was also effective, which cured 60% of the mice, While transferring LAK cells or 'LAK-88/DC' cells at the same time with L615 inoculation or 1 day after L615 inoculation presented no apparent therapeutic effect. However, when combined with cyclophosphamide (CY) chemotherapy, the transfer of LAK cells or 'LAK-88/DC ' cells 5 days after L615 inoculation could prolong the mean survival time and even cured 25%-28.6% of the advanced L615 leukemic mice.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1990年第5期296-300,共5页
Chinese Journal of Immunology
基金
国家"七.五"攻关专题及辽宁省教委重点课题经费资助
关键词
LAK细胞
细胞系
抗癌
LAK cell
LAK-88/DC cell line
Adoptive immunotherapy
L615 murine leukemia
