摘要
BANYULS(BAN)基因编码的花青素还原酶是缩合单宁合成有关的一个关键酶。利用同源克隆的原理,采用RACE的方法,从紫花苜蓿中克隆得到了BAN基因(MsBAN),并对其进行了初步的分析。该基因cDNA全长1313bp,包括开放阅读框1017bp,编码339个氨基酸,在N端存在一个NADP结合位点"VIGGTGFVASL-LIKQLLEKGY"。实时荧光定量PCR结果表明,该基因在紫花苜蓿荚果中表达量最高,花中最弱。在NaCl和PEG诱导下,MsBAN基因表达量明显高于对照。在外源激素ABA和GA3处理下,该基因被诱导表达。表明单宁可能在紫花苜蓿的抗逆性调控中也起到一定的作用。
BANYULS（BAN）gene,encoding the anthocyanidin reductase（ANR）,is a key enzyme in condensed tannin synthesis pathway.Based on the homologous gene sequence method,a cDNA of BAN gene was cloned from Medicago sativa by using rapid amplification of cDNA ends（RACE） method.The full-length of cDNA sequence is 1313 bp and includes a 1017bp open reading frame（ORF） which encodes a 339-amino-acid polypeptide.A NADP binding site（VIGGTGFVASLLIKQLLEKGY） was observed in the N-terminal region of deduced amino acid sequence of MsBAN.The results of Real-time PCR indicated that the expression level of BAN gene was highest in pods,and least in flower.The expression of BAN gene is up-regulated by NaCl and PEG stress.Under exogenous hormones ABA and GA3 treatments,the gene expression was significantly induced.In conclusion,condensed tannin possibly plays a role in response to abiotic stress in Medicago sativa.
出处
《中国草地学报》
CSCD
北大核心
2012年第2期8-15,共8页
Chinese Journal of Grassland
基金
中国农业科学院基本科研业务费项目(2011cj-16)
国家"十二五"科技支撑项目(2011BAD17B01)资助
关键词
紫花苜蓿
BAN基因
克隆
生物信息学分析
表达分析
Medicago sativa
Anthocyanidin reductase
Cloning
Bioinformatic analysis
Expression analysis
