摘要
目的建立NaOH乙醇溶液和γ射线辐照两步法灭活壳聚糖中病毒的工艺。方法以牛病毒性腹泻病毒(Bovine viral diarrhoea virus,BVDV)和猪细小病毒(Porcine parvovirus,PPV)为指示病毒,分别采用NaOH乙醇溶液和γ射线辐照法灭活壳聚糖中的BVDV和PPV,并对两种方法进行优化。以优化的灭活条件建立两步法灭活工艺,测定病毒滴度、壳聚糖相对分子质量、脱乙酰度和乙醇含量。结果含8 mol/L NaOH的10%乙醇溶液,35℃处理1 h后冻干,再采用γ射线辐照(5 kGy),BVDV和PPV的滴度均下降4 lgTCID50以上,同时,壳聚糖的相对分子质量仅降低8.4%,脱乙酰度无显著变化,未检出残留乙醇。结论 NaOH乙醇溶液和γ射线辐照两步法可有效灭活壳聚糖中的指示病毒,同时对壳聚糖质量影响较小,为保证壳聚糖作为生物材料及药用辅料使用的安全性提供了保障。
Objective To develop an inactivation procedure for virus in chitosan by sodium hydroxide ethanol solution and irradiation. Methods Bovine viral diarrhea virus （BVDV） and porcine parvovirus （PPV） as indicators in ehitosan were inactivated by sodium hydroxide ethanol solution and ~/ irradiation separately, and the two methods were optimized. A two-step inactivation procedure was developed under optimized condition, by which the inactivated samples were determined for virus titer, relative molecular mass of chitosan, degree of deacetylation and ethanol content. Results After treatment with 10% ethanol solution containing 8 mol/L sodium hydroxide at 35~C for 1 h followed by lyophilization and irradiation with ~/-ray at a dosage of 5 kGy, both BVDV and PPV titers in chitosan decreased by more than 4 lgTCIDs0, while the relative molecular mass of chitosan decreased by only 8. 4%, the degree of deacetylation showed no significant change, and no residual ethanol was detected. Conclusion The two-step inactivation procedure consisting of treatment with sodium hydroxide ethanol solution and 7 irradiation was effective for inactivation of indicator viruses in chitosan, while showed little effect on chitosan, which ensured the safety of ehitosan as a biomaterial and a medicinal subsidiary material.
出处
《中国生物制品学杂志》
CAS
CSCD
2012年第4期502-505,509,共5页
Chinese Journal of Biologicals
基金
国家"863"计划项目(2007AA091603)
江苏省科技成果转化专项资金项目(BA2009082)
食品科学与工程国家重点实验室年度目标导向项目(SKLF-MB-200805)
关键词
壳聚糖
病毒灭活
氢氧化钠
乙醇
Γ射线
Chitosan
Virus inactivation
Sodium hydroxide
Ethanol
y Ray
