摘要
【目的】探讨肉鸡肺小动脉中膜5-羟色胺(5-HT)含量与肺小动脉平滑肌细胞增殖的关系,明确5-羟色胺转运体(5-HTT)是否参与了肉鸡肺动脉高压的发生。【方法】20日龄科宝肉鸡分成非诱病组(静脉注射生理盐水,包括空白对照组、氟西汀、西酞普兰组,30只/组)和诱病组(静脉注射纤维素微粒,包括药物空白对照组、氟西汀组、西酞普兰组,50只/组)。自21d起,记录肺动脉高压综合征(PHS)发病率,并分别于21、28、35、42d从各组随机抽样,测定右心室/全心室重量比(RV/TV),采用免疫组织化学技术、增殖细胞核抗原(PCNA)染色和核仁组织区嗜银蛋白(AgNOR)染色方法检测肺小动脉中膜5-HT量及肺小动脉平滑肌细胞增殖情况。【结果】诱病组PHS发病率、RV/TV明显高于非诱病组(P<0.05),肺小动脉中膜5-HT量增加,平滑肌细胞上嗜银颗粒增多,PCNA阳性细胞增多,而两种5-HTT抑制剂(氟西汀和西酞普兰)能明显减少肉鸡PHS发病率和肺小动脉中膜5-HT量,抑制平滑肌细胞增殖,但两种药物之间无明显差异。【结论】在肉鸡肺动脉高压发生过程中,由5-HTT介导的肺小动脉平滑肌细胞内5-HT增多,引起肺小动脉平滑肌细胞过度增殖,导致肉鸡PHS发生,5-HTT可能是防治肉鸡PHS的有意义靶分子。
【Objective】 An experiment was designed to detect the relationship between the content of 5-hydroxytryptamine（5-HT） in the media layer of pulmonary arterioles and the proliferation of pulmonary arterioles smooth muscle cells,with the purpose to clarify whether or not 5-HT transporter（5-HTT） was participated in the occurrence of pulmonary hypertension in broilers.【Method】Broilers at 20 day of age were randomly divided into non-disease inducing groups（intravenously injection of normal saline,including blank control group,Fluoxetine group,Citalopram group,30 fowls in each group） and disease inducing groups（intravenously injection of cellulose particles,including medicine blank control group,Fluoxetine group,Citalopram group,50 fowls in each group.）.From day 21 on,the morbidity of pulmonary hypertension syndrome（PHS） of each group were recorded.Samples were randomly taken from each group on day 21,28,35 and 42,and the weight ratio between right ventricle and total ventricle（RV and TV） was determined.5-HT content in pulmonary arteriolar media layer was detected by using the immunohistochemical technique,and the proliferation conditions of smooth muscle cells in pulmonary arterioles was detected by argyrophilic nucleolar organizer region proteins staining method（AgNOR） and proliferating cell nuclear antigen（PCNA） staining method.【Result】PHS morbidity and RV/TV value of disease-inducing groups were significantly higher than that of non-disease inducing groups（P0.05）.The 5-HT content in pulmonary arteriolar media was increased in disease-inducing groups,as well as the argyrophilic particles on the smooth muscle cells and the PCNA positive cells.Fluoxetine and Citalopram（both are 5-HTT inhibitors） significantly reduced the morbidity of PHS and 5-HT contents in pulmonary arteriolar media in broiler,and inhibited the proliferation of smooth muscle cells.There were no significant difference between the two medicines.【Conclusion】During the occurrence of pulmonary hypertension in broilers,the increasing of 5-HT content in pulmonary arteriolar smooth muscle cells which was mediated by 5-HTT resulted in the excessive proliferation of smooth muscle cells,leading to the occurrence of PHS.5-HTT is possibly a significant target molecular for the prevention and treatment of PHS in broilers.
出处
《中国农业科学》
CAS
CSCD
北大核心
2012年第6期1191-1198,共8页
Scientia Agricultura Sinica
基金
国家自然科学基金资助项目(30600440)
关键词
肺动脉高压
平滑肌细胞
5-羟色胺
5-羟色胺转运体抑制剂
肉鸡
pulmonary hypertension
smooth muscle cells
5-hydroxytryptamine
5-hydroxytryptamine transporter inhibitor
broiler
