摘要
用循环伏安法和线性扫描伏安法研究了头孢氨苄在碳糊电极上的电化学行为,考察了不同电解质溶液、pH以及扫描速率等的影响。实验表明:在2.0 mol·L-1HCl支持电解质中,头孢氨苄的降解产物在-0.45 V(vs.SCE)处的电化学还原反应为2电子与2质子参加的受吸附控制的不可逆过程。还原峰电流与头孢氨苄的浓度的平方根在1.8×10-8～3.0×10-4mol·L-1范围内呈良好的线性关系,回收率在95.7%～101.5%范围,检出限(S/N=3)为1.0×10-8mol·L-1。并探讨了头孢氨苄在电极上的反应机理。
In this work,we studied the electrochemistry characteristic of cefalexin at a carbon paste electrode by using cyclic voltammetry（CV） and linear sweep voltammetry（LSV）.The influence factors such as the electrolyte solution,pH values of the support solution and the scanning rate were discussed.Results showed that the electrochemical behavior of cefalexin hydrolysate exhibited irreversible absorption-controlled redox process of two electrons and two protons in the solution of 2.0 mol·L-1HCl,with irreversible reductive peak at-0.45 V（vs.SCE）.The peak currents were proportional to the square root of cefalexin concentration in the range of 1.8×10-8~3.0×10-4 mol·L-1 with a detection limit of 1.0×10-8 mol·L-1.The range of recovery was 95.7%~101.5%.The reaction mechanism of cefalexin at the carbon paste electrode was discussed.
出处
《分析科学学报》
CAS
CSCD
北大核心
2012年第2期182-186,共5页
Journal of Analytical Science
基金
国家863计划(No.2009AA03Z331)
国家自然科学基金(No.20875081)
关键词
头孢氨苄
碳糊电极
线性扫描伏安法
Cefalexin
Carbon paste electrode
Linear sweep voltammetry
