摘要
目的：制备PGA／β-磷酸三钙（β—TCP）支架并研究其物理性能。方法：以PGA与β—TCP为材料，六氟异丙醇（HFIP）为溶剂，以氯化钠颗粒（颗粒尺寸：425～500μm）为制孔剂，按PGA与β～TCP质量比1：1（A组）与1：3（B组），采用改良溶液浇铸一离子沥滤的方法制备PGA／β-TCP骨组织工程复合支架材料。借助扫描电镜对其微结构进行观察，并测试不同质量比的PGA／β-TCP骨组织工程支架复合材料的物理、力学性能。结果：B组0．13gPGA／0．39gp—TCP支架材料的抗压缩强度大于A组0．13gPGA／0．13gB—TCP，B组抗压缩强度值为（731．283±55．214）kPa，A组为（173．833±36．140）kPa，差异有统计学意义（P〈0．05）。A、B组支架材料孔隙率均大于85％，A组孔隙率（93．1±0．7）％大于B组的（89．3＋0．6）％，差异有统计学意义（P〈0．05）。SEM照片可见2组支架材料均为多孔三维架构，孔径均为200～300μm，孔隙的连通性好。β-TCP均匀地分散于聚合物中，B组支架材料中β-TCP颗粒明显多于A组。结论：质量比1：3的PGA／β-TCP支架材料有望成为一种理想的骨组织工程支架材料。
Objective： To fabricate porous PGA/β-tricalcium phosphate （15-TCP） for tissue engineering scaffolds and test its properties. Methods： Using PGA andβ-TCP as scaffold materials, 1,1,1,3,3,3-hexafluoro-2-propanol （HFIP） as solvent, sodium chloride as porogen （particle size： 425-500μm）, the PGA/β-TCP, in 1 ： 1 （A group） and 1 ： 3 （B group） weight ratios, scaffolds for tissue engineering were fabricated by means of solvent-casting particulate leaching. The microstructures of the scaffolds were observed using scanning electron microscopy （SEM）. The physical and mechanical properties were tested in A and B groups. Results： The compressive strength was significantly higher in B group （0.13 g PGA/0.39 g （3-TCP） than that of A group （0.13 g PGA/0.13 g β-TCP, P 〈 0.001）. The compressive strength after surgery were（731.283±55.214）kPa and （ 173.833±36.140）kPa in A group and B group. There was a high porosity in both A and B groups （〉85%）. The opening porosi- ties was significantly higher in A group（93.1 ±0.7）% than that of B group（89.3 ±0.6）. The SEN pictures showed irregular micro-pores in both groups. The average pore size was 200-300 pore in two groups. 13-TCP particles were uniformly dispersed within the PGA matrix. There were more β-TCP granules in B group than that of A group. Conclusion： The PGA/β-TCP scaffold material（weight ratio = 1：3 ） might be a promising scaffold for bone tissue engineering.
出处
《天津医药》
CAS
北大核心
2012年第5期496-498,共3页
Tianjin Medical Journal
基金
天津市卫生局科技基金项目(项目编号:2010ky20)
