摘要
目的:从中药材楮树皮中筛选出抑制α-糖苷酶的物质,并考察其对糖分吸收的抑制作用。方法:建立α-糖苷酶抑制剂的体外筛选模型,对多种楮树皮提取物中的α-糖苷酶抑制物质进行筛选;建立大鼠小肠外翻囊体外模型,探讨所筛出的抑制物质对蔗糖吸收的抑制作用。结果:从楮树皮乙酸乙酯提取物中筛选出了两个有抑制作用的生物碱Broussonetinine B(B1)和Broussonetine(B2),它们对蔗糖吸收的半数抑制浓度(IC50)分别为0.530 mg.mL-1、0.445 mg.mL-1。结论:本筛选模型简便可靠,为开发中药楮树皮的新用途奠定了理论基础。
Objective：To screen out α-glucosidase inhibitors from the Chinese herbs Broussonetia papyrifera（L.） Vent.,and to investigate their inhibitions on sugar absorption.Methods：An α-glucosidase inhibitor screening model was established in vitro.It was used to screen some extracts of Broussonetia papyrifera（L.） Vent.,the reverted gut sac method was established.The inhibitions of the screened out substances on sugar absorption were investigated with the method.Results： Two alkaloids with inhibition,Broussonetinine B（B1） and Broussonetine（B2） were obtained from the bark of Broussonetia papyrifera（L.） Vent.Their IC50 on absorption of sugar were 0.530mg·mL-1,0.445mg·mL-1 respectively.Conclusion：The model is convenient and reliable.The results will provide a theoretical principle for development of new application of the bark of Broussonetia papyrifera（L.） Vent.
出处
《中华中医药学刊》
CAS
2012年第4期711-712,共2页
Chinese Archives of Traditional Chinese Medicine
基金
中央高校基本科研业务费专项资金资助项目(SWJTU09ZT28)
四川省应用基础研究项目(2007J13-059)
关键词
Α-糖苷酶抑制剂
楮树
体外筛选
外翻肠囊
α-glucosidase inhibitor
Broussonetia papyrifera（L）vent
screening in vitro
reverted gut sac
