摘要
目的探讨用中和试验检测低水平HbsAg的临床意义。方法用酶联免疫吸附试验(ELISA)或化学发光免疫分析法(CLIA)仪检测为HBsAg弱阳性的标本,再用HBsAg中和试剂盒(酶联免疫法)进行检测,并结合相应样本的肝功能及HBV-DNA检测结果进行分析。结果经ELISA法初筛为弱阳性的35例样本中,中和试剂盒检测为阳性27例、阴性8例。经CLLIA法检测为弱阳性的10例样本中,中和试剂盒检测为阳性4例,阴性6例。结论低水平HbsAg病例中和试验抑制率值越大,肝功能异常率越高,HBV-DNA阳性率也越高。
Objective To discuss the significance of low level HBsAg neutralization test. Methods Weak positive reaction with HBsAg Serum samples by ELISA or CLIA were detected by using neutralization test,the results will becomparsed to the results of liver function tests and HBV--DNA tests. Results Among 35 samples that showed weak positive reaction with ELISA,27 samples showed positive in neutralization test,but the other 8 samples show negative.Among 10 samples that showed weak positive reaction with CLIA,4 samples showed positive in neutralization test and the other 6 samples showed negative.Conclusion The larger the suppression rate of neutralization test,the higher rates of liver dysfunction and positive rates of HBV-DNA.
出处
《中外医疗》
2012年第10期160-160,162,共2页
China & Foreign Medical Treatment
关键词
乙型肝炎病毒表面抗原
中和试验
Hepatitis B virus Surface antigen
Neutralization test
