摘要
通过TCBS培养基分离筛选对虾养殖水体中的细菌,采用16S rDNA分子生物学方法和Biolog微生物自动分析系统进行鉴定分析。结果显示:以TCBS培养基分离筛选的60株菌中,采用16S rDNA分子生物学方法鉴定,只有SWG-27、SWG-28、SWG-29为弧菌属,占总鉴定细菌的5%,其他细菌大部分为芽孢杆菌属(Bacillus sp.)和大洋芽孢杆菌属(Oceanobacillus sp.),分别占总鉴定细菌的36.7%和43.3%;自60株细菌中随机挑选15株作Biolog微生物自动分析系统鉴定,弧菌属细菌也只占很小比例;60株细菌接种于6种不同品牌的TCBS培养基上均可很好地生长。结果表明,TCBS培养基能够培养养殖水体中的弧菌,但其他细菌也能在其上良好生长,利用TCBS培养基检测养殖水体弧菌数量的结果值得商榷。
Using TCBS culture medium,some bacteria were isolated and screened from Litopenaeus vannamei culture ponds.,and they were identified by 16S rDNA and Biolog identification system.The results of 16S rDNA showed that among 60 strains,SWG-27,SWG-28,SWG-29 were Vibrio sp.These only accounted for 5% of all the identified bacteria.Most of them were Bacillus sp.and Oceanobacillus sp.,which accounted for 36.7% and 43.3%.We selected 15 strains from the 60 bacteria,with Biolog we knew that most of the 15 bacteria were not Vibrio sp..Only one bacteria was Vibrio sp.,others were Bacillus sp.,Pseudomonas sp.,etc.And all the bacteria we selected could grow well on 6 different TCBS culture mediums.The results demonstrated that Vibrio sp.could be cultivated using TCBS culture medium.However,other bacteria also could grow well on TCBS culture medium.Therefore,it is worth to discuss on using TCBS medium to test the quantity of Vibrio sp..We could use molecular method and biology method to identify Vibrio sp.accurately.
出处
《广东农业科学》
CAS
CSCD
北大核心
2012年第7期141-144,共4页
Guangdong Agricultural Sciences
基金
国家现代农业(虾)产业技术体系建设专项(nycytx46)
国家公益性行业(农业)科研专项(201103034)
国家"十二五"科技支撑计划项目(2011BAD13B10)
广东省科技计划项目(2010B02039001)
南海水产研究所中央级公益性科研院所基本科研业务费专项(2010YD05)
广东省鱼病防治专项(2130108)
