摘要
细菌脂肪酶是一类重要的工业用酶,其分泌系统有着严谨的机制。革兰阳性细菌利用Sec-转运系统使脂肪酶跨过质膜完成分泌;革兰氏阴性细菌的外泌蛋白通过Sec-转运系统、Tat-转运系统或其他机制跨越内膜后,还必须利用Ⅰ型、Ⅱ型、Ⅲ型、Ⅳ型与Ⅴ型分泌系统来完成跨外膜分泌。详细介绍细菌脂肪酶分泌主要依赖的Sec-或Tat-跨内膜的转运系统及革兰氏阴性细菌的Ⅰ型、Ⅱ型与Ⅴ型自分泌系统的3种不同分泌方式。细菌脂肪酶分泌的研究对人们认识其分泌机制,并利用基因工程的手段提高其外泌产量等具有重要的指导意义。
Lipases represent the most important class of enzymes used in industry.Lipases secretion across the inner membrane of both gram-positive and gram-negative organisms generally follows the same routes,involving the Sec-dependent pathway or twin-arginine translocation（Tat）pathways.Translocation across the gram-negative inner membrane results in release of the protein into the periplasmic space.Therefore,gram-negative bacteria have evolved I-V five dedicated secretion systems in order to achieve translocation of these proteins to the cell surface and beyond.In this review,we comprehensively describe the Sec-dependent pathway,Tat translocation pathways,type I secretion system,type II secretion system and type V secretion system involved in the lipases secretion.The researches on lipase secretion provide vital sources for improving the secretion production of bacterial lipases by the genetic engineering methods.
出处
《生物技术通报》
CAS
CSCD
北大核心
2012年第4期27-34,共8页
Biotechnology Bulletin
基金
国家自然科学基金项目(30900839)
关键词
细菌脂肪酶
蛋白分泌
Ⅰ型分泌系统
Ⅱ型分泌系统
Ⅴ型自分泌系统
Bacteria lipase Protein secretion Type I secretion system Type II secretion system Type V secretion system
