摘要
目的建立高效液相色谱测定血清中左乙拉西坦(抗癫痫病)浓度的方法。方法用Hypersil C18色谱柱,流动相为乙腈-水(6∶94),流速为0.8 mL.min-1,检测波长210 nm。血清样品上SPE柱,以含20%乙腈溶液作洗脱液,收集洗脱液直接进样测定。结果左乙拉西坦血清样品线性范围为0.7～100.0mg.L-1,8.93,35.71,71.42 mg.L-13种浓度方法回收率分别为102.6%,95.7%,98.1%,日内、日间RSD均小于7.5%。结论本方法准确性好、灵敏度高、操作简便,可满足左乙拉西坦血药浓度监测和药代动力学研究的要求。
Objective To establish a HPLC method for determination of the concentration of levetiracetam in serum.MethodsThe analysis was conducted with Hypersil C18 column.Acetonitrile-water was served as mobile phase,the flow number was 0.8 mL·min-1,and the detecting wavelength was at 210 nm.Serum sample was applied to SPE column.ResultsThe linear range of levetiracetam in serum was 0.7-100.0 mg·L-1.The recovery rate of low concentration,moderate concentration and high concentration（8.93,35.71,71.42 mg·L-1） was 102.6%、95.7%、98.1%,respectively.Both of the intra-day and inter-day RSD were less than 7.5%.ConclusionThis method is accurate,sensitive and simple.And it is suitable for the determination of the concentration of levetiracetam in serum and the pharmacokinetic study of levetiracetam.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2012年第4期289-291,共3页
The Chinese Journal of Clinical Pharmacology
