摘要
采用胚胎肢芽细胞进行原代微团培养 ,培养物经不同浓度的五氧化二钒 (V2 O5 )对细胞肢芽细胞经 5天增减形成细胞集落 ,V2 O5 对体外肢芽细胞的分化有明显抑制作用 ,并有较明显的剂量 -效应关系 ,其半数生长抑制浓度(ICV5 0 )和半分化抑制浓度 (ICD5 0 )分别为 12 .6和 1.6μg ml,V D值为 7.9。表明抑制细胞分化可能是V2 O5
The micromass rat embryo Limb bud culture was used to study the effect of Vandium pentoxide(V_2O_5) on neuron differentiation.The results showed that the limb bud cell was differntiated after 5 day in micromass culture the V 2O 5 obviously inhibited the differentiation,and proliferation of limb bud cell invitro test and dose-dependent response was observed.The ICV 50 and (ICD 50 ) values for foci formation and cell survival were 1.6 and 12.6μg/ml, specificaly.It is suggested that V_2O_5 specificaly inhibits the differentiation of limb bud cell. \ \
出处
《镇江医学院学报》
2000年第1期13-15,共3页
Journal of Zhenjiang Medical College
关键词
五氧化二钒
肢芽细胞
发育毒性
细胞分化
Vandium pentoxide
Limb bud cell
Cell developmental toxicity
In vitro
