摘要
鸭坦布苏病毒是新发现的一种可引起鸭产蛋下降、生长迟缓和死亡的黄病毒。目前尚无可靠的快速诊断方法用于该病毒的检测。本研究根据鸭坦布苏病毒E基因序列,设计了2对重叠引物P1、P2和P3、P4,建立了检测鸭坦布苏病毒的套式RT-PCR方法,该套式RT-PCR比一般PCR敏感性高10倍。采用该方法对安徽省、河北省、山东省、浙江省、上海市等地发病鸭场的63份样品进行了检测,阳性检出率为48/63(76.2%),而病毒分离率仅为13/63(20.6%)。由此表明,与其他方法相比较,本试验所建立的套式RT-PCR方法具有快速、敏感、特异优点,可用于鸭坦布苏病毒的流行病学调查及病毒的检测。
The duck Tembusu virus,a newly emerging Flavivirus,caused egg production decline,retarded growth,and even death among ducks in China's Mainland.However,there was no reliable,rapid and sensitive method to detect the virus.According to the sequence of the E gene,two pairs of primers P1,P2 and P3,P4 were designed,and a nested RT-PCR assay was established to detect the duck Tembusu virus.The results showed that the sensitivity of the nested RT-PCR was ten times higher than that of the general PCR.To test the reliability of nested RT-PCR assay,sixty-three clinical samples collected from ducks in Anhui,Hebei,Shangdong,Zhejiang,and Shanghai were tested.Forty eight samples were duck Tembusu virus positive by this method,while only thirteen of these samples were positive by virus isolation.The result indicated that the nested RT-PCR assay was a sensitive,specific and rapid diagnosis method for detecting duck Tembusu virus.
出处
《中国动物传染病学报》
CAS
2011年第3期34-37,共4页
Chinese Journal of Animal Infectious Diseases
基金
公益性行业(农业)专项经费资助(201003012)
农业部动物转基因专项(2009ZX08010-022B)
