摘要
目的 研究氟中毒对大鼠肝、肾组织磷脂脂肪酸组成的影响。方法 选用 Wistar纯系大鼠 ,饲以高浓度含氟水 1个月 ,来复制慢性氟中毒动物模型。用改良 Falch方法提取和分离动物肝、肾组织磷脂 ,用气相色谱法分离和测定磷脂各类脂肪酸构成。结果 所有饲以高浓度氟水的动物均有不同程度的氟斑牙形成 ,尿氟含量增高 ,肝和肾组织蛋白质含量降低等慢性氟中毒的表现 ;氟中毒动物肝、肾组织磷脂脂肪酸组成发生异常改变 ,表现为多不饱和脂肪酸减少 ,饱和脂肪酸组成增加 ,减少的多不饱和脂肪酸种类是花生四稀酸和二十二碳六烯酸。结论 表明长期摄入过量氟可导致肝、肾组织磷脂脂肪酸组成改变 。
Objective To study the influence of experimental fluorosis on fatty acid composition of phospholipid in rat liver and kidney.Methods Wistar rats were fed with various amounts of fluoride in drinking water for seven months to produce the animal model with chronic fluorosis.Phospholipid in rat liver and kidney was extracted by the improved Falch method and fatty acid compositions of this lipid were analysed using gas chromatography.Results The results showed that all animals fed with high amount of fluoride suffered from chronic fluorosis,which manifested with dental fluorosis,the increase of fluoride level in urine,and the decrease of protein content in liver and kidney as compared to controls.The abnormal composition of fatty acids from phospholipids in liver and kidney was observed in the rats with chronic fluorosis.The proportion of unsaturated fatty acids was decreased and the saturated fatty acids increased.The species of unsaturated fatty acids influenced by fluorosis were 20:4 (arachidolic acid) and 22:6.Conclusions The study suggests that high amount of fluoride can induce modification of fatty acid compositions of phospholipid in liver and kidney,which might be the result from oxidative stress and the increased level of lipid peroxidation. [
出处
《中国地方病学杂志》
CAS
CSCD
2000年第1期22-25,共4页
Chinese Jouranl of Endemiology
基金
国家自然科学基金!3 92 60 0 2 8
关键词
氟中毒
肝
肾
磷脂
脂肪酸
大鼠
Chronic fluorosis
Liver
Kidney
Phospholipid
Fatty acid
