摘要
目的探讨神经中间丝蛋白NF-66能否作为判断胰岛素瘤良恶性的分子标志物。方法采用免疫组织化学染色法检测胰岛素瘤组织、瘤旁胰腺组织及3株胰岛素瘤细胞株NF-66蛋白的表达，应用单因素及多因素统计分析胰岛素瘤NF-66蛋白表达与肿瘤病理特征及患者预后的相关性。结果132例胰岛素瘤组织中102例（77％）表达NF-66蛋白，98例瘤旁胰腺组织均未见该蛋白表达（P=4．86×10^-3。）；3株胰岛素瘤细胞株均高表达NF-66蛋白。118例良性胰岛素瘤中96例（81％）表达NF-66，而14例恶性胰岛素瘤中6例（43％）表达NF-66，恶性胰岛素瘤的表达显著下降（P=0．003）。胰岛素瘤NF．-66表达与肿瘤大小（以3cm为界）、远处转移、远处并淋巴结转移均呈显著负相关（46％比81％，P=0．009；38％比81％，P=0．013；43％比81％，P=0．010）；而与患者性别、年龄、肿瘤复发和生存状态不相关。结论胰岛素瘤中NF-66表达下调与肿瘤的恶性生物学特征有关，可能成为潜在的鉴别胰岛素瘤良恶性的分子标志物。
Objective To investigate whether neuronal intermediate filament protein （NF-66） could be used as a molecular marker for the determination of malignancy of insulinoma. Methods The expression of NF-66 protein was detected in insulinoma and para - cancerous tissues and 3 insulinoma cell lines by immunohistochemistry staining. The relationship between the expression of NF-66 protein and the clinicopathological characteristics, survival was analyzed by univariate and multivariate statistical analysis. Results Expression of NF-66 was found in 102（77% ） out of 132 insulinomas and none of 98 paired control （P = 4.86 × 10^-31 ）. NF-66 was highly expressed in 3 insulinoma cell lines. The expression of NF-66 was found in 96 （81%） out of 118 benign tumors （P =0.003）, while out of 14 malignant insulinomas, 6（43% ） were found to express NF-66 （P = 0.003 ）. The expression of NF-66 was significantly associated with tumor size （3 cm as the cut-off point）, distant metastasis （38% vs 81%, P =0.013） and distant plus lymph node metastasis （46% vs 81%, P =0.009）, respectively. The expression of NF-66 was not correlated with age, gender, recurrence and overall survival. Conclusions Down-regulation of NF-66 was significantly associated with tumor malignancy, suggesting that NF-66 could be a potentially novel molecular biomarker to distinguish malignancy from benign insulinoma.
出处
《中华胰腺病杂志》
CAS
2012年第2期89-91,共3页
Chinese Journal of Pancreatology
基金
国家自然科学基金（30850002、8107205）
北京协和医院卫生部内分泌重点实验室专项基金
关键词
胰岛素瘤
中间丝蛋白质类
诊断
鉴别
预后
Insulinoma
Intermediate filament proteins
Diagnosis, differential
Prognosis
