摘要
目的探索适用于Pythiumsp.GY1938菌株简并PCR的基因组DNA模板制备方案。方法采用微波法、石英沙法、氯化苄法、CTAB法、尿素法提取DNA,并通过特异PCR和简并PCR评价各种方案的优弊。结果 5种方法制备的DNA含量差异无统计学意义(F=2.355,P>0.05);DNA制备效率相近;特异性PCR结果一致,但氯化苄法制备的DNA经简并PCR扩增出的条带较多,目的条带更清晰。结论 5种方法均适于特异性PCR所需DNA模板的制备,但氯化苄法更适于Pythiumsp.GY1938菌株简并PCR模板DNA的制备。
Objective To explore the best method for degenerate primer PCR extraction of genomic DNA from the Pythium sp.GY1938 strain.Methods Five methods were used to extract genomic DNA and their efficiency was separately evaluated with specific and degenerate primer PCR.Results Results of specific primer PCR showed that there were no significant differences in the five methods.However,results of degenerate primer PCR showed that the benzyl chloride method was more effective because it a larger number of clearer bands.Conclusion The benzyl chloride method was best suited to degenerate primer PCR extraction of genomic DNA from the Pythium sp.GY1938 strain.
出处
《中国病原生物学杂志》
CSCD
北大核心
2012年第3期192-194,共3页
Journal of Pathogen Biology
基金
四川省教育厅青年基金项目(No.10ZB095)
成都医学院创新性实验项目(No.CX2010015)和综合性
设计性实验项目(No.ZH2010003)
关键词
真菌
基因组
简并
Fungi
genomic DNA
degenerate
