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Effect of Heat Treatment Combined with Shoot Tip Culture on the Virus-Free of Arena Strawberry 被引量:1

Effect of Heat Treatment Combined with Shoot Tip Culture on the Virus-Free of Arena Strawberry
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摘要 With Arena strawberry as experimental materials, heat treatments combined with shoot tip culture were used to explore their detoxification effects. The results indicated that if not heated, splitting shoot tips directly could not completely remove viruses. On the other hand, it will be obviously improved after shoot tips are heated. Incubators heat treatment (inconstant temperature) with shoot tip culture, constant temperature water bath incubation process with meristem-tip culture, hot air treatment (constant temperature) with apical meristem culture were three methods tested in searching for virus-free of Arena strawberry in this experiment. And their virus-free rates were 78.9%, 86.0% and 50.0% respectively. Of the three methods, the second, constant temperature water bath process with shoot tip culture, with 78.7% survival, is the best. Thus, it can be inferred that constant temperature water bath treatment combined with shoot tip culture might be the most appropriate method to remove viruses of Arena strawberry.
出处 《Journal of Life Sciences》 2012年第2期168-174,共7页 生命科学(英文版)
关键词 Arena strawberry heat treatment shoot tip virus-free. 茎尖培养 清除病毒 竞技场 热处理 草莓 茎尖分生组织培养 免费 恒温水浴
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