摘要
目的 研究ＰＣＲ－ ＳＳＣＰ 检测肺癌、肠癌中ｐ５３ 基因突变的方法改进及突变率。方法 用ＰＣＲ－ＳＳＣＰ，银染技术检测４ 例肺癌和７ 例大肠癌组织标本中ｐ５３ 基因外显子５ ～８ 。结果 肺癌ｐ５３ 基因突变率为７５％ （３／４ 例），肠癌ｐ５３ 基因突变率为４２．９％ （３／７ 例） 。结论 ｐ５３ 基因突变与肺癌、肠癌的发生密切相关，ＰＣＲ－ＳＳＣＰ银染技术简便快速、灵敏安全的基因突变检测方法，可应用于肿瘤的临床基因诊断。
Purpose The frequency of p53 gene mutation was studied by using PCR-SSCP method and the method we used was evaluated.Method We detected the mutation of exons 5-8 in the p53 gene of 4 lung cancers and 7 colorectal carcinomas by using silver dye PCR-SSCP method.Results The frequency of p53 gene mutation of lung cancers and colorectal carcinomas were 75%(3 of 4) and 42.9%(3 of 7),respectively.Conclusion The mutation of p53 gene is highly related with lung cancer and colorectal carcinoma and the silver dye PCR-SSCP is a handy,fast,safe and sensitive detection method which can be used in gene diagnosis of tumors.
出处
《苏州医学院学报》
2000年第1期42-44,共3页
Acta Academiae Medicinae Suzhou
基金
苏州医学院青年基金
