摘要
采用RT-PCR法扩增出中国境内生长的琉璃苣Δ6-脂肪酸脱氢酶基因,序列分析表明,该基因全长为1 359个核苷酸,与美国德克萨斯州的琉璃苣Δ6-脂肪酸脱氢酶基因同源性可达到99%。将克隆到的基因连接pRTL2质粒的35S启动子,构建重组载体pPTN-D6D,并通过农杆菌介导的子叶节转化系统,将该基因导入到垦农18和小粒豆2个大豆品种中。经PCR检测分析,初步证明琉璃苣Δ6-脂肪酸脱氢酶基因已导入并整合到大豆基因组中。
γ-linolenic acid is an essential polyunsaturated fatty acid in human metabolism.Δ6-fatty acid desaturase（D6D）is the key enzyme that can convert linoleic acid into γ-linolenic acid in vivo.In this study,the Δ6-fatty acid desaturase gene from Borago officinalis was amplified by RT-PCR.Sequence analysis showed that the full cDNA contained 1 359 nucleotides.The homology of Borago officinalis D6D gene in China and that registered in Texas USA was 99%.Recombinant vector pPTN-D6D was constructed that containing the Δ6-fatty acid desaturase gene from Borago officinalis and 35S promoter from plasmid pRTL2.The gene was transferred into two soybean cultivars,Xiaolidou and Kennong18,with Agrobacterium mediated cotylendon node transformation system.The results of PCR indicated that the D6D gene had been introduced and integrated into soybean genome which laid a foundation for soybean quality improvement.
出处
《大豆科学》
CAS
CSCD
北大核心
2012年第2期173-177,共5页
Soybean Science
基金
北京市教委科技发展计划项目(KM2009011008)
北京市自然科学基金项目(6112003)
