摘要
选用野生大豆、栽培大豆按异黄酮含量不同(高、中、低)配制杂交组合,对33个组合进行杂种优势分析。结果表明:中亲优势为正向优势的组合为15个,占全部组合的45.5%。具有超高亲优势的组合为12个,其中高异黄硐含量母本组合5个,占全部正向超高亲优势的41.7%,说明高异黄酮亲本杂种优势明显。18个栽培与野生杂交组合中超高亲优势正向优势组合为11个,中亲优势正向优势组合为13个,说明栽培与野生杂交组合的杂种优势明显,但14个栽培×野生杂交组合F2优势降低,表现为自交衰退,因此获得高异黄酮的后代材料仍需进一步选择。
In order to analyze heterosis of isoflavone content in interspecific hybridization,33 hybridized combinations were obtained by 15 accessions of Glycine max and Glycine soja which were different in isoflavone content（high,middle and low）.Isoflavone concentrations of F1,F2 as well as their parents were determined by high performance liquid chromatography.Fifteen combinations which accounted for 45.5% of total had positive mid-parent heterosis.Twelve combinations showed positive over-parent heterosis,among which,five combinations had higher isoflavone content in female parent.Among 18 combinations between Glycine max and Glycine soja,11 had positive over-parent heterosis and 13 had mid-parent heterosis.Results suggest heterosis of isoflavone content were obvious when parents with higher isoflavone content were selected or interspecific hybridization were conducted.Heterosis of isoflavone content in 14 interspecific hybridization combinations showed inbreeding depression in F2 generation.Therefore,it is essential to do further selection in following generations for high isoflavone breeding.
出处
《大豆科学》
CAS
CSCD
北大核心
2012年第2期203-207,共5页
Soybean Science
基金
公益性行业(农业)科研专项经费(2000903040)
转基因生物新品种培育重大专项(2011ZX08004-003)
公益性行业(农业)科研专项经费项目(200903007-09)
