期刊文献+

基于SRAP的赣南油茶良种分子鉴别研究 被引量:24

SRAP-based molecular identification of elite cultivars of Camellia oleifera from south Jiangxi province
下载PDF
导出
摘要 油茶是我国南方的主要木本食用油料树种,赣南油茶良种是我国重要的油茶资源组成部分。利用SRAP(序列相关扩增多态性)标记技术,对我国油茶良种分子鉴别进行探索性研究。以14个赣南油茶良种为对象,提取叶片基因组DNA作为模板,筛选出稳定性高的引物组合;采用SRAP-PCR的优化反应体系,对获得的清晰可重复的条带进行{0,1}数据转换,并根据SRAP的谱带特征建立了赣南油茶良种的分子鉴别体系,为油茶良种的分子鉴定提供了理论依据和技术基础。 Camellia oleifera is one of main tree species of woody edible oil in South China,and important elite cultivars resource in southern Jiangxi province.The researches on molecular identification of the elite cultivars in south Jiangxi were carried out by SRAP(sequence related amplification polymorphism).By Using the leaf genomic DNA extracted from 14 elite Camellia oleifera cultivars in south Jiangxi province,the improved CTAB method as template,the high-stable primer groups were selected out based on the improved SRAP-PCR system,which can bring specific DNA bands,good polymorphism and high stability.According to the SRAP-based band pattern from these primer groups,the polymorphic DNA bands were transformed in range of {0,1} data,which was used to establish the primary system of molecular identification of elite cultivars from south Jiangxi,thus providing theoretic basis and technique foundation of molecular identification of Chinese elite Camellia oleifera cultivars.
出处 《中南林业科技大学学报》 CAS CSCD 北大核心 2012年第3期147-151,共5页 Journal of Central South University of Forestry & Technology
基金 国家"十一五"科技支撑计划课题(2009BADB1B02 2009BADB1B10)
关键词 赣南油茶 分子鉴别 SRAP 良种 Camellia oleifera molecular identification SRAP elite cultivars
  • 相关文献

参考文献23

  • 1邹锋,谭晓风,袁德义,袁军.RAPD分子标记及其在油茶遗传育种研究中的应用(综述)[J].亚热带植物科学,2009,38(1):70-73. 被引量:11
  • 2张党权,田华,谢耀坚,黄青云,谷振军,曹家光,谭晓风,曾艳玲,邓顺阳,樊绍刚.桉树4个种遗传多样性的ISSR分析[J].中南林业科技大学学报,2010,30(1):12-17. 被引量:24
  • 3Li G,Quiros C F.Sequence-related amplified polymorpgism(SRAP)a new marker system based on a simple PCR reaction:its application to mapping and gene tagging in Brassica[J].Theor.Appl.Genet.,2001,103:455-461.
  • 4Riaz A,Li G,Quresh Z,et al.Genetic diversity of oilseedBrassica napus lines based on sequence-related amplifiedpolymorphism and its relation to hybrid performance[J].PlantBreeding,2001,120(5):411-415.
  • 5郭大龙,张君玉,李猛,张国海,刘崇怀.葡萄SRAP反应体系优化及引物筛选[J].基因组学与应用生物学,2010,29(2):379-384. 被引量:35
  • 6Ferriol M,Pico B,Nuez F,et al.Genetic diversity of agermplasm collection of Cucurbita pepo using SRAP and AFLPmarkers[J].Theor.Appl.Genet.,2003,107(2):271-282.
  • 7Yu M Y,Ma B,Luo X,et al.Molecular Diversity of Auriculariapolytricha Revealed by Inter-Simple Sequence Repeat andSequence-Related Amplified Polymorphism Markers[J].Curr.Microbiol.,2008,56:240-245.
  • 8祝全东,张党权,李晓云,杨书斌,韩欣,宋志丹,刘作梅,赵倩,谢晓敏,邝先松.油茶SRAP标记的PCR体系建立与优化[J].中南林业科技大学学报,2010,30(3):57-62. 被引量:21
  • 9Primmer C R,Ellgren H.Patterns of molecular evolution in avianmicrosatellite[J].Mol.Biol.Evol.,1998,15(8):997-1008.
  • 10Gunasekare M T K.Applications of molecular markers to thegenetic improvement of Camellia sinensis L.(tea)-A review[J].The Journal of Horticultural Science&Biotechnology,2007,82(2):161-169.

二级参考文献168

共引文献178

同被引文献470

引证文献24

二级引证文献177

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部