摘要
目的探讨孕妇在不同甲状腺疾病及自身免疫状态时，胎盘Ⅱ型和Ⅲ型碘化甲状腺原氨酸脱碘酶（D2、D3）表达的变化。方法选择处于不同甲‘状腺功能及自身免疫状态的孕妇为研究对象，并以既往无甲状腺疾病及相关疾病史、妊娠期甲状腺功能正常且抗甲状腺过氧化物酶抗体（TPOAb）阴性的孕妇作为对照，用RT．PCR法对所获得的足月产孕妇的胎盘组织进行D2、D3的检测，观察表达的变化。结果胎盘组织中同时存在D2和D3的表达。在甲减未控制组产妇胎盘中，D2的相对灰度值为0．916±0．035，高于正常对照组的0．833±0．029（P〈0．05）；而D3的相对灰度值低于正常对照组（0．766±0．038与0．848±0．052），但差异无统计学意义（P〉0．05）。妊娠前或妊娠中有甲亢或甲减的孕妇，经过有效治疗，分娩前甲状腺功能已控制在正常范围的产妇，其胎盘D2及D3的表达与正常对照组比较差异无统计学意义（P均〉0．05）。甲状腺功能正常但TPOAb阳性的产妇，其胎盘D2和D3的相对灰度值分别为0．842±0．032、0．837±0．053，与正常对照组相比差异均无统计学意义（P均〉0．05）。结论人类胎盘组织中D2和D3并存。在母体甲减时，胎盘中D2的表达增强，D3的表达有所减弱，这种变化可能对稳定母体与胎儿间甲状腺激素输送起有益的作用。
Objective To investigate the expression of type Ⅱ and type Ⅲ iodothyronine deiodinases （D2 and D3 ） of human placenta on pregnant women with different thyroid diseases and different autoimmunity. Methods Pi＇egnant women with different thyroid diseases and autoimmunity were selected into the experiment group, and pregnant women who had no individual or family history of thyroid diseases with normal thyroid function and negative thyroid peroxidase antibody （TPOAb）were selected into the control group. Expression level of iodothyronine deiodinase（ D2 and D3 ） on placenta was measured by RT-PCR. Results D2 and D3 were both expressed on human placenta. D2 showed significantly higher expression level in hypothyroidism uncontrolled group than that in control group （0. 916 ±0. 035 vs 0. 833± 0. 029, P 〈 0. 05 ）, however, D3 showed a lower expression（0. 766± 0. 038 vs 0. 848 ± 0. 052）, on placenta and the difference was not significant（P 〉 0.05 ）. In those who had history of hyperthyroidism or hypothyroidism but whose thyroid function become normal by effective treatment, D2 and D3 expression exhibited no difference from that of the control（P 〉 0. 05）. In those pregnant women with normal thyroid function and TPOAb-positive, D2 and D3 expression was（0. 842 ±0. 032）and（0. 837 ±0. 053 ）respectively and there was also no difference from that of the control（P 〉 0. 05）. Conclusion D2 and D3 were simultaneously expressed on human placenta. In those pregnant women with hypothyroidism, the level of D2 expression is high and the level of 133 expression is low. Those changes might be important and helpful for the stabilization of thyroid hormone transportation between mother and fetus.
出处
《中国综合临床》
2012年第5期462-466,共5页
Clinical Medicine of China
基金
上海市卫生局科技发展基金（054017）
