摘要
目的和方法 :X射线照射结合无血清培养诱导小鼠脾细胞凋亡 ,采用流式细胞术定量分析碱性成纤维细胞生长因子 (bFGF)对脾细胞凋亡的抑制作用 ,并采用淋巴细胞转化试验 [3H]-TdR掺入法检测bFGF对脾细胞的增殖效应。结果 :小鼠经过不同剂量X射线照射 ,脾细胞再经过不同时程无血清培养 ,都出现了典型的亚二倍体Ap峰 ,1μg/mL和 2 μg/mL的bFGF均可降低Ap峰中的细胞数 (细胞凋亡率 ) ,并能显著增强脾细胞的增殖反应能力。结论 :bFGF可抑制外周免疫器官脾细胞的凋亡 ,并促进其增殖 。
AIM: To investigate the effect of basic fibroblast growth factor (bFGF) on radiation-induced splenocytic apoptosis of mice. METHODS: At 14 h after whole body irradiation with 0.5 Gy and 1.0 Gy,splenocytes were cultured with and without bFGF,and splenocytic apoptosis was quantitatively analysed by flow cytometry. Cell proliferation was determined by the method of [3H]-TdR incorporation. RESULTS: bFGF(1 μg/mL and 2 μg/mL) could reduce the rate of cell apoptosis ,and promote the proliferation of splenocytes. CONCLUSION: bFGF could inhibit radiation-induced splenocytic apoptosis and promote the proliferation of splenocytes and then enhance body immunity.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2000年第2期131-134,共4页
Chinese Journal of Pathophysiology
基金
国家"九五"攻关资助项目! (96 -C0 2 - 0 1- 0 3)
