摘要
目的:探讨亚麻木酚素(SDG)联合蛋白酶体抑制剂硼替佐米(Bortezomib,Bor)对肺腺癌A549细胞凋亡的影响及其机制。方法:MTT法检测细胞增殖;Annexin V-FITC/PI双染流式细胞法和Hoechst 33258荧光染色法检测细胞凋亡;比色法检测半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)活性;Real Time PCR检测Caspase-3、BCL-2、BAXmRNA的表达;Western Blot检测BCL-2、Bax及p-JNK的蛋白表达。结果:SDG联合Bor能明显抑制细胞生长,上调Caspase-3活性,促进p-JNK、BAX mRNA和蛋白的表达,抑制BCL-2 mRNA和蛋白的表达,诱导细胞凋亡。结论:SDG联合Bor可显著诱导肺腺癌A549细胞的凋亡,其机制可能与其激活JNK信号通路有关。
Objective： To study the influence of Secoisolariciresinol Diglucoside（SDG） combined with Bortezomib on induction of apoptosis in Lung Cancer Cell Line A549 and its relative mechanisms.Methods： The effect on proliferation was evaluated by MTT assay.The cell apoptosis was studied by flow cytometry and Hoechst 33342 staining.Colorimetric method was used to detect the activity of Caspase-3.Real Time PCR was used to detect the expression of Caspase-3,BCL-2 and BAX mRNA.Western blot was used to determine the change of p-JNK,BCL-2 and BAX protein expression in A549 cells.Results： The cell growth was significantly slowed down and the cell apoptosis was induced after the combined treatment.Meanwhile the Caspase-3 activity and the expression of Caspase-3 mRNA were obviously increased,the expression of BCL-2 mRNA and protein were significantly down regulated and the expression of BAX.p-JNK mRNA and protein were significantly up regulated after the combined treatment.Conclusion： The results demonstrate that SDG combined with Bortezomib can significantly induce apoptosis of A549 cells,its mechanisms may be involved in activation of the JNK pathway.
出处
《中药材》
CAS
CSCD
北大核心
2012年第2期248-253,共6页
Journal of Chinese Medicinal Materials
关键词
亚麻木酚素
硼替佐米
肺腺癌A549细胞
细胞凋亡
Secoisolari ciresinol Diglucoside
Bortezomib
Lung Cancer Cell Line A549
Apoptosis
