摘要
目的:比较研究红芪替换玉屏风口服液中的黄芪后对免疫抑制小鼠细胞免疫调节作用。方法:通过给小鼠腹腔注射免疫抑制剂环磷酰胺致成免疫功能低下模型,以相同剂量的红芪替代玉屏风口服液中的黄芪灌胃进行体内拮抗实验,测定胸腺指数、脾脏指数、巨噬细胞吞噬功能、T淋巴细胞的增殖能力、杀伤活性、T细胞亚群、血清IL-1β的含量。结果:玉屏风口服液和红芪替换黄芪的玉屏风水提物均可显著提高被环磷酰胺抑制的小鼠胸腺指数和脾脏指数;使巨噬细胞吞噬功能明显增加;并能拮抗环磷酰胺对小鼠脾脏T淋巴细胞增殖、杀伤活性、T细胞亚群数量和IL-1β产生的免疫抑制作用。且红芪替换黄芪的玉屏风水提物提高被环磷酰胺抑制的小鼠脾脏指数和T淋巴细胞增殖能力优于玉屏风口服液。结论:红芪与防风、白术配伍后与玉屏风口服液相比在细胞免疫调节方面具有相似的作用,且在恢复脾脏质量和T淋巴细胞增殖方面优于玉屏风口服液。
Objective：To study on Radix Hedyseri as substitute for Radix Astragali of Yupingfeng oral liquid on cellular immunity in immunosuppressed mice.Methods：The model of immunosuppression mice were induced by Cyclophosphamide.And the same dose of Radix Hedyseri and Radix Astragali alternative Yupingfeng oral liquid was intragastric administrated into mice;Antagonistic experiments were observed in vivo.Determined the thymus gland index,spleen index,phagocytosis of the macrophage,proliferation index of T lymphocyte,kill and wound activity,T lymphocyte subgroup,and content of IL-1β of serum.Results：Yupingfeng oral liquid and Yupingfeng aqueous extract of Radix Hedyseri substitute Radix Astragali both could significant raise thymus gland index and spleen index,and clearly increase the phagocytosis of the macrophage.They both could antagonize immunosuppressive action caused by Cyclophosphamide,which could promote T lymphocyte proliferation,kill and wound activity,quantity of T lymphocyte subgroup,and production of IL-1β with different degree.And Yupingfeng aqueous extract of Radix Hedyseri substitute Radix Astragali increased spleen index and T lymphocyte proliferation was better than those of Yupingfeng oral liquid.Conclusion：Radix Hedyseri and Radix Saposhnikoviae,compatible with Rhizoma Atractylodis Macrocephalae compared with Yupingfeng oral liquid in cell immunity regulation has a similar role,and better in the recovery of spleen weight and T cell proliferation.
出处
《中药材》
CAS
CSCD
北大核心
2012年第2期269-273,共5页
Journal of Chinese Medicinal Materials
基金
中国博士后科学基金资助(201003073)
教育部高等学校博士点基金项目(200807330006)
关键词
红芪
黄芪
玉屏风口服液
免疫抑制
细胞免疫
Radix Hedyseri
Radix A stragali
Yupingfeng oral liquid
Immunosuppression
Cytoimmunity
