摘要
为了阐明乙醇中毒对神经系统损害的机理 ,用0 .5- 5.0 g· L-1浓度的乙醇处理原代培养的 CD新生大鼠大脑皮层星形细胞 ,用反转录 PCR和Northern印迹杂交方法同时测定神经营养因子( NGF)的基因表达水平 .结果显示 0 .5g·L-1乙醇在染毒 2 4 h后 ,NGF m RNA水平开始降低 ,5.0 g· L-1在染毒 48或 72 h后 ,NGF m RNA水平与对照组比较降低了 50 % - 58% .结果表明 ,乙醇中毒在体外可降低新生大鼠星形细胞 NGF m RNA的生成 ,这种作用可干扰神经元与胶质细胞间的正常交互作用并可能与胚胎发育期乙醇接触导致的神经病理改变有关 .
In order to elucidate the mechanism of ethanol impairment on nerve system, primary cultures of astrocytes from the cerebral cortex of neonatal rats were exposed to ethanol at concentrations of 0.5-5.0 g·L -1 . The levels of nerve growth factor(NGF) mRNA were analyzed and quantified by reverse transcription polymerase chain reaction(RT PCR) and Northern blot methods. The results showed that the levels of NGF mRNA were decreased significantly by exposure to the ethanol at the concentration as low as 0.5 g·L -1 for 24 h. The highest concentration of ethanol at 5.0 g·L -1 produced 50% and 58% reduction of NGF expression after 48 and 72 h incubation compared to untreated cultures. Our results indicate that ethanol inhibits NGF synthesis of astrocytes in vitro. The reduction of NGF may lead to the disruption of neuronal glia interaction and underlie, in part, the neuropathology associated with fetal ethanol exposure.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2000年第2期140-143,共4页
Chinese Journal of Pharmacology and Toxicology
关键词
乙醇
基因表达
神经生长因子
星形细胞
ethanol
gene expression
nerve growth factors
astrocytes
cells, cultured
