摘要
目的探讨乌司他丁(UTI)预处理对小鼠创伤性脑水肿潜在的治疗作用及其机制。方法BALB/c小鼠38只,随机分为生理盐水对照组(control组)和乌司他丁预处理组(UTI组)。采用测干湿重法检测不同剂量UTI预处理对小鼠脑组织含水量的影响和Western-blot检测脑组织中水通道蛋白4(AQP4)蛋白表达情况。结果与生理盐水对照组比较,UTI能明显减轻神经功能障碍,干湿重法检测结果显示,随着UTI剂量的增大小鼠脑组织含水量明显降低,Western-blot结果提示UTI下调AQP4蛋白的表达,不同组间差异有统计学意义(P<0.05)。结论乌司他丁预处理能减轻创伤性脑水肿,具有显著的神经保护作用,可能是通过调控AQP4而减轻脑组织水肿。
Objective To investigate the potential therapeutic effect and the related mechanism of ulinastatin （UTI） on traumatic brain edema in mice. Methods Thirty-eight BALB/e mice were randomly divided into two groups： isotonic Na chloride control group （control group） and UTI pretreatment group （UTI group）. The wet-dry weighing method was performed to measure the brain water content of mice. The expression of aquaporin 4 （AQP4） was detected by Western blot. Results Compared with control group, UTI significantly inhibited the neurological dysfunction of mice in a concentration-dependent manner and reduced brain water content. It is suggested that UTI could down-regulate the expression of AQP4 （ P 〈 0. 05 ）. Conclusion Pretreatment of UTI can effectively protect traumatic brain edema, which may be associated with the regulation of AQP4 expression.
出处
《中华神经外科疾病研究杂志》
CAS
2012年第2期113-116,共4页
Chinese Journal of Neurosurgical Disease Research
基金
国家自然科学基金资助项目(30930093)
天普研究基金资助项目(01200908)
