摘要
目的 :观察 5-羟色胺 ( 5- HT)对耳蜗微循环的影响。方法 :在豚鼠圆窗膜置放 2 μl0 .5%的5- HT,以激光多普勒流量仪 ( LDF)测量耳蜗微循环的 3个参数 :血流量 ( CBF)、运动的血细胞的数目 ( CMBC)、血流速度 ( Veloc)的变化 ;以压力传感器测量平均动脉血压 ( MABP)的变化。结果 :耳蜗微循环出现了两个时期的变化 :第一时期迅速下降形成陡峰 ,各参数的峰值为 CBF:86.8% ,CMBC:90 .2 % ,Veloc:96.3% ;第二时期呈持续平稳变化 ,其中 CBF、CMBC持续平稳地下降 ,Veloc无明显变化。耳蜗微循环的平均变化为 CBF:89.7% ;CMBC:89.6% ;Veloc:1 0 0 .1 %。MABP无显著变化。结论 :5- HT能减少耳蜗供血 ,导致耳蜗微循环紊乱。
Objective:The present study was undertaken to investigate the influence of serotonin (5 HT) on cochlear microcirculation.Method:2 μl 0.5 % 5 HT were applied to round window membrance in anaesthetized guinea pigs.The three parameters as blood flow (CBF),number of blood cell moving (CMBC) and blood velocity (Veloc) of cochlear microcirculation were measured with laser doppler flowmeter.The mean arterial pressure (MABP) was recorded simultaneously.Result:5 HT induced a biphasic response of reduction in cochlear blood flow.In the first phase,cochlear microcirculation showed a transient abruptly reduction.The CBF,CMBC and Veloc reached to 86.8 %, 90.2 % and 96.3 % of the baseline respectively.The second phase reduced slowly.The CBF and CMBC decreased to 89.7 % and 89.6 % by average throughout the experiment period respectively.The Veloc and MABP had no significant changes.Conclusion:5 HT could decrease the cochlear blood flow and result in cochlear microcirculation dysfunction.
出处
《临床耳鼻咽喉科杂志》
CSCD
2000年第2期81-82,共2页
Journal of Clinical Otorhinolaryngology
基金
卫生部优秀科技人才专项基金!(1995-144号)
国家教委留学回国人员科技基金!(1996-644号)
关键词
血清素
耳蜗
微循环
多普勒流量仪
LDF
Serotonin Cochlear Microcirculation Laser doppler flowmeter
