摘要
目的 :探讨戊型肝炎患者血清 HEV - RNA的动态变化及其意义。方法 :用逆转录 -巢式聚合酶链反应检测 32例戊型肝炎患者系列血清 HEV- RNA。结果 :发病后 0～ 7d,8～ 14d,15～ 2 1d,2 2～ 2 8d患者血清 HEV-RNA阳性率分别为 96 .6 % ,84.4% ,43.8%和 9.4%。病后 1周内 ,血清 HEV- RNA阳性率明显高于抗 - HEV Ig M(P <0 .0 5 )和抗 - HEV Ig G阳性率 (P <0 .0 1)。病后 2周后 ,抗 - HEV Ig M和抗 - HEV Ig G阳性率均明显高于血清HEV- RNA阳性率 (P <0 .0 1)。结论 :大部分戊型肝炎患者血清 HEV- RNA阳性持续至病后 2周 ,少部分持续至病后 3周以上。病后 1周内 ,检测血清 HEV- RNA作为早期诊断指标最敏感。但病后 2周由于血清 HEV- RNA部分阴转 ,抗 - HEV阳性率增加 ,作为诊断指标抗 - HEV比 HEV- RNA敏感。
Objective: This paper was to explore the dynamics of HEV RNA in the sera of patients with hepatitis E and its significance. Methods: Reverse transcription nested polymerase chain reaction (RT nested PCR) was used to detect HEV RNA in serial sera of 32 patients with hepatitis E. Results: From the first week to the fourth week after onset of illness the positive rate of HEV RNA in the sera was 96.6%, 84.4%, 43.8%, and 9.4% respectively. In the first week of the onset, the positive rate of HEV RNA was higher than those of anti HEV IgM (P<0.05) and anti HEV IgG (P<0.01). After two weeks of the onset the positive rates of both anti HEV IgM and anti HEV IgG were higher than that of HEV RNA (P<0.01). Conclusions: HEV RNA lasted for two weeks after onset of illness for most patients and more than three weeks for a few patients. Within the first week of illness course, detecting HEV RNA in the sera was most sensitive for early diagnosis. After the second week of the course, detecting anti HEV was more sensitive for diagnosis than detecting HEV RNA.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2000年第2期137-139,共3页
Journal of China Medical University
基金
卫生部科研基金!资助项目
913 12 2 93
关键词
戊型肝炎
戊型肝炎病毒
RAN
聚合酶链反应
RT nested PCR
hepatitis E
hepatitis E virus
RNA
anti HEV IgM
anti HEV IgG
