摘要
目的探讨应用高脂饮食建立慢性系膜增殖性肾炎血管病变模型的方法。方法雄性Wistar大鼠行单侧肾切除后随机分为单纯肾切除组、单纯肾炎组、单纯高脂组、肾炎高脂组。单纯肾炎组、肾炎高脂组在单侧肾切除后3 d尾静脉注射OX7抗体(100 mg/kg),1周后尾静脉连续注射OX7抗体(每次100 mg/kg,1次/周,共3次),单纯肾切除组和单纯高脂组在同一时间尾静脉注射PBS,注射抗体后第2天单纯高脂组、肾炎高脂组腹腔注射维生素D3(6万U/kg,1次/4周),同时给予高脂饲料。分别于第4、8、10周观察各组大鼠的一般情况、体重、血压、尿蛋白、血浆白蛋白、血脂、血钙、肾功能以及肾脏病理改变。结果模型组(肾炎高脂组)大鼠第8周肾小球外的小动脉出现管壁增厚,管腔变小,平滑肌细胞减少,细胞排列紊乱,纤维组织增生。第10周单纯肾炎组和单纯高脂组肾小球外小动脉管壁轻度增厚,管腔变化不明显,模型组血管病变积分明显高于单纯肾炎组和单纯高脂组(P<0.05)。结论通过对慢性抗Thy1肾炎大鼠加用高脂饲料并腹腔注射维生素D3的方法,可以成功建立慢性系膜增殖性肾炎血管病变模型。
Objective To establish a rat model of progressive mesangial proliferative glomerulonephritis with vasculopathy by high fat diet feeding.Methods Male Wistar rats were subjected to unilateral nephrectomy and the surviving rats were randomly assigned into 4 groups： high fat diet group,nephritis group,nephritis fed with high fat diet group,and unilateral nephritis rats severed as controls.OX7（100 mg/kg）antibody was firstly injected into the tail vein of rats of the nephritis group and nephritis fed high fat diet group at 3 days after nephrectomy,and then injected once per week for 3 times.The rats of nephritis group and high fat diet group received saline injection.Vitamin D3 was injected intraperitoneally in a dose of 600 000 U/kg to the rats at the second day after OX7 antibody injection,once per week for 4 weeks and fed with hgh fat diet.The general status was recorded and the body weight,systolic blood pressure,proteinuria,serum total protein,serum albumin,triglyceride,cholesterol,serum creatinine and serum calcium were measured.The renal function was assessed and renal alterations were observed by histopathology.Results Intrarenal arterial wall thickening,arterial stenosis and proliferation of fibrous tissue were observed in the rats of nephritis and high fat diet group at week 8.At week 10,the intrarenal arterial wall was slightly thickened in the rats of nephritis and high fat diet group.The scores of glomerular,tubulointerstitial and vascular histological damages in the rats of nephritis with high fat diet group were significantly higher than those in the rats of nephritis group and high fat diet group（P0.05）.Conclusions Animal model of progressive mesangial proliferative glomerulonephritis with vasculopathy can be successfully established by high fat diet plus intraperitoneal injection of vitamine D3 in chronic anti-Thy1 nephritis rats.
出处
《中国实验动物学报》
CAS
CSCD
2012年第2期64-69,F0003,共7页
Acta Laboratorium Animalis Scientia Sinica
关键词
高脂
系膜增殖性肾炎
血管病变
High fat diet
Mesangial proliferative glomerulonephritis
Vasculopathy
Rats
