摘要
以D380为载体进行脯氨酰内肽酶的固定化研究,确定了其最佳固定化条件为加酶量为140U/g,戊二醛浓度为0.3%,固定化温度为25℃,吸附14h,交联6h。固定化酶活达到89.5U/g,酶活回收率63.9%。固定化酶最适反应温度为40℃,最适反应pH值为5.5。
Using the macroporous resin of D380 as the immobilized support,immobilization of proline specific endoprotease was studied.The results showed that the optimal conditions for immobilization were as following： crude enzyme 140U/g,glutaraldehyde volume fraction 0.3%,immobilization temperature 25℃,absorption time 14h,crosslinking time 6h.The activity remained of immobilized proline specific endoprotease achieved 63.9% in average and the activity of immobilized enzyme was 89.5U/g.The optimum temperature of immobilized enzyme was 40℃,and the optimum pH of immobilized enzyme was pH 5.5.
出处
《中国酿造》
CAS
2012年第4期114-117,共4页
China Brewing
基金
天津市质量技术监督局科技开发基金项目(11-02)
关键词
脯氨酰内肽酶
固定化
大孔树脂
proline specific endoprotease
immobilization
macroporous resin
