摘要
编码枯草芽孢杆菌(Bacillus subtilis strain GD3b)木聚糖酶(Xylanse B,Xyn B)基因从重组载体pUC-XynB中切割,并克隆到pPICZa A载体中,经酶切和测序鉴定,成功构建了分泌型毕赤酵母表达载体pPICZa-Xyn B。载体经线性化后转入毕赤酵母X-33,并筛选获得可通过甲醇诱导分泌表达Xyn B重组蛋白的毕赤酵母工程菌X-33/Xyn B。
The Bacillus subtilis strain xylanse B（Xyn B）gene was digested from the recombinant plasmid pUC-Xyn B and cloned into vector pPICZa A.After restriction enzyme digestion and sequencing identifications,the secreted expression pichia vector pPICZa-Xyn B was constructed successfully.The recombinant vector pPICZa-Xyn B was linearized and transformed into Pichia pastoris X-33 to rebuild the Pichia pastoris recombinant strain X-33/Xyn B,which could be secreted express Xyn B recombinant fusion protein with methanol induction.
出处
《中国饲料》
北大核心
2012年第9期19-21,共3页
China Feed
基金
广西青年基金项目资助(桂科青0991068)
关键词
木聚糖酶
毕赤酵母
表达
重组蛋白
xylanase
Pichia pastoris
expression
recombinant protein
