摘要
通过EMS诱变籼稻品种IR64获得一个稳定遗传的淡褐色斑点叶突变体lbsl1(light brown spotted leaf 1)。在自然条件下,突变体播种后10～14d,叶片上出现淡褐色斑点,随后逐渐扩散至全叶,第1叶至剑叶上均有淡褐色斑,为全生育期性状。斑点性状的表达对株高、生育期、结实率和千粒重等农艺性状具有显著的影响。遗传分析结果表明,该淡褐色斑点叶性状受一个隐性核基因控制。将突变体lbsl1与正常叶色水稻Morobereken杂交构建F2定位群体,利用SSR标记,最终将该淡褐叶基因lbsl1(t)定位在第6染色体短臂上一个约130kb的区段上。定位的结果和发展的群体为该基因的进一步精细定位和克隆奠定了基础。
A light brown spotted-leaf mutant of rice was isolated from an EMS-induced IR64 mutant bank. The mutant, designated as lbsll （light brown spotted-leaf 1 ）, displayed light brown spot in the whole growth period from the first leaf to the flag leaf under natural summer field conditions. Agronomic traits including plant height, growth duration, seed-setting rate and 1000-grain weight of the mutant were significantly affected. Genetic analysis showed that the mutation was controlled by a single recessive gene, tentatively termed lbsll （t）, which was mapped to the short arm of chromosome 6. With developed SSR markers, the gene was finally delimited to an interval of 130 kb between markers RM586 and RM588. The lbsll （t） gene is likely a novel rice spotted-leaf gene since no other similar genes have been i- dentified near the chromosomal region. The genetic data and recombination populations provided will facilitate further fine-mapping and cloning of the gene.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2012年第3期297-301,共5页
Chinese Journal of Rice Science
基金
水稻生物学国家重点实验室自主课题(ZZKT200801)
国家863计划资助项目(2011AA10A101)
中央级公益性科研院所专项基金资助项目
关键词
水稻
叶色
斑点叶突变体
分子标记
基因定位
rice
leaf color spotted-leaf mutant molecular marker
gene mapping
