摘要
建立离子色谱法同时分离检测两种水果和两种饮品中的蔗糖、葡萄糖和果糖的分析方法。采用METROSEPCARB 1(150mm×4.0mm)阴离子交换分离柱和脉冲安培检测器,对淋洗分离条件进行优化。确定柱温20℃、淋洗液30mmol/L NaOH溶液,淋洗液流速1.0mL/min,总分析时间25min。在1.0～50.0mg/L范围内蔗糖、葡萄糖和果糖分别呈良好线性。在优化的分离条件下,蔗糖、葡萄糖和果糖的检出限分别为0.085、0.126mg/L和1.072mg/L(进样体积20μL,峰面积定量)。用8.0mg/L蔗糖、葡萄糖和果糖的混标溶液连续5次进样,3种糖峰面积的相对标准偏差分别为6.09%、2.90%和3.03%,保留时间的相对标准偏差分别为2.49%、1.78%和1.79%。样品测定的回收率为83.4%～126.6%。用该方法检测了水果和饮品中的3种糖,效果良好。同时具有操作简单,结果准确,快速有效等优点,可为水果和饮品质量检测提供一个简单有效的方法。
An ion chromatographic method has been developed for the determination of sucrose,glucose and fructose in two kinds of fruits and two kinds of drinks.The analytes were separated on a METROSEP CARB 1（150 mm × 4.0 mm） anion exchange column using 30.0 mmol/L NaOH as mobile phase at flow rate of 1.0 mL/min and detected with a pulsed amperometric detector.The total time it took to separate the sugars was 25 min.The linear ranges of sucrose,glucose and fructose were 1.0-50.0 mg/L.The detection limits for sucrose,glucose and fructose were 0.085,0.126 mg/L and 1.072 mg/L（20μL injection,and the quantitative analysis was based on the peak areas）,respectively.The RSDs of peak areas for five consecutive determinations of 8 mg/L sucrose,glucose and fructose were 6.09%,2.90% and 3.03%,respectively.The RSDs of retention time were 2.49%,1.78% and 1.79%,respectively.The recovery rates for the sugars across three spike levels were in the range of 83.4%-126.6%.Satisfactory results were achieved for the determinations of sucrose,glucose and fructose by the method.Meanwhile,the method was simple,accurate,fast,effective and applicable for the quality control of fruits and drinks.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第8期159-162,共4页
Food Science
基金
国家科技重大专项(2008ZX07315)
重庆市自然科学基金项目(CSTC
2007BB5370)
关键词
离子色谱
脉冲安培检测
蔗糖
葡萄糖
果糖
ion chromatography
pulsed amperometric detection
sucrose
glucose
fructose
