摘要
建立高效阴离子交换色谱-脉冲安培检测法测定奶粉中葡萄糖、蔗糖和乳糖的方法。以METROSEP CARB1(150mm×4.0mm)阴离子交换柱为分离柱,脉冲安培检测,37mmol/L NaOH溶液为淋洗液,以柠檬酸作为蛋白质沉淀剂。葡萄糖、蔗糖和乳糖的线性范围分别为1～40、1～50、1～50mg/L,相关系数分别0.9966、0.9968、0.9985,检出限分别为0.014、0.091、0.083mg/L,精密度为1.10%～4.96%,回收率为90.13%～104.83%。该方法分析时间短,前处理简单,适用于快速测定奶粉中的葡萄糖、蔗糖和乳糖。
A high performance anion exchange chromatographic-pulse amperometric detection method was established to determine glucose,sucrose and lactose in milk powder.The separation column used was METROSEP CARB 1（150 mm × 4.0 mm） anion exchange column,and the mobile phase was 37 mmol/L NaOH.Citric acid was used to precipitate proteins from milk.The linear ranges of glucose,sucrose and lactose were 1-40,1-50 mg/L and 1-50 mg/L with correlation coefficients of 0.9966,0.9968 and 0.9985,respectively,and the detection limits were 0.014,0.091 mg/L and 0.083 mg/L.The precision relative standard deviations of precision for 5 replicate determinations were in the range of 1.10%-4.96%.The recovery rates for glucose,sucrose and lactose in 4 different commercial samples were 90.13%-104.83%.The method was suitable for rapid determination of glucose,sucrose and lactose in milk powder,thanks to its short analysis time and simple pretreatment.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第8期176-179,共4页
Food Science
基金
重庆市自然科学基金项目(CSTC
2007BB5370)
国家科技部重大专项(2008ZX07315)
关键词
高效阴离子交换色谱
脉冲安培检测
奶粉
葡萄糖
蔗糖
乳糖
柠檬酸
high performance anion exchange chromatography
pulsed amperometric detection
milk powder
sugar
citric acid
