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p-JNK在结肠癌组织中的表达及抑制JNK通路后HT-29细胞增殖和凋亡变化 被引量:1

Expression of p-JNK in colon cancer and its effect on proliferation and apoptosis of human colon cancer HT-29 cells
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摘要 目的研究p-JNK在结肠癌组织中的表达以及抑制JNK信号通路后人结肠癌HT-29细胞增殖和凋亡的变化。方法使用免疫组织化学方法检测50例结肠癌组织标本和50例正常结肠组织标本中p-JNK的表达情况并和临床资料进行比较分析;在人结肠癌细胞株HT-29中使用SP600125抑制JNK信号通路后,MTT法检测细胞增殖,TUNEL法检测凋亡。结果 p-JNK在结肠癌组织中的表达水平明显高于正常对照组织(P<0.05);并和淋巴结转移、肿瘤分化程度、肿瘤的侵犯深度相关(P<0.05);而与肿瘤部位无关。抑制JNK信号通路后,HT-29细胞中的p-JNK表达降低(P<0.05);增殖减少,凋亡增加(P<0.05)。结论在结肠癌组织中存在p-JNK的高表达,抑制JNK信号通路能降低人结肠癌细胞株HT-29细胞的增殖,并促进凋亡;JNK信号通路和结肠癌的发生发展有关。 Objective To study p-JNK expression in colon cancer tissues and the effects of JNK signal pathway on proliferation and apoptosis of Colon cancer HT-29 cells.Methods Immunohistochemical methods were applied to study p-JNK expression in colon cancer tissues and the association between the expression and clinical pathological data such as colon cancer staging and lymph node metastasis.Colon cancer HT-29 cells were divided into two groups,normal control group and JNK inhibitor group(cells were pretreated by SP600125 with 24h),MTT method was used to detect the cell proliferation,and TUNEL method was used to detect the apoptosis.Results The p-JNK expression in the colon cancer tissue was higher than that of the control tissue(P0.05) and the expression level was correlated with the condition of lymph node metastasis,degree of tumor differentiation and depth of tumor invasion(P0.05).After inhibiting the JNK signaling pathway using the JNK inhibitor,p-JNK expression was reduced in human colon cancer HT-29 cells(P0.05) and proliferation of HT-29 cells was decreased in a dose-dependent manner(P0.05),and apoptosis was increased(P0.05).Conclusions The p-JNK are highly expressed in human colon cancer tissues and correlated with the degree of tumor differentiation and lymph node metastasis.Inhibit JNK signal pathway in colon cancer HT-29 cells can significantly inhibit the cell proliferation and promote cell apoptosis.
出处 《医药论坛杂志》 2012年第4期15-18,21,共5页 Journal of Medical Forum
关键词 JNK 结肠肿瘤 HT-29细胞 JNK Colonic neoplasms HT-29 cells
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参考文献10

  • 1Heasley IrE, Han SY. JNK regulation of oncogenesis[ J]. Mol Cells, 2006, 21(2) : 167-173.
  • 2Hibi M, Lin A, Smeal T, et al. Identification of an oncoprotein- and UV - responsive protein kinase that binds and potentiates the c - Jun activation domain [ J ]. Genes Dev, 1993,7 ( 11 ) : 2135-2148.
  • 3NateriAS, Spencer - DeneB, BehrensA. Interaction of phospho- rylated c - Jun with TCF4 regulates intestinal cancer develop-ment[ J]. Nature,2005,437(7056) :281-285.
  • 4She QB, Chen N, Bode AM, et al. Deficiency of c - Jun - NH ( 2 ) - terminal kinase - 1 in mice enhances skin tumor develop- ment by 12 - O - tetradecanoylphorbol - 13 - acetate[J]. Canc- er Res ,2002,62 ( 5 ) : 1343-1348.
  • 5周燕红,何小飞,高卉,王瑶芬.JNK信号通路在大肠癌侵袭和转移中的作用及机制[J].世界华人消化杂志,2009,17(21):2142-2146. 被引量:12
  • 6王晓艳,沈守荣,刘芬,彭娅,李桂源,范松青.结肠癌中NGX6抑制EGFR/K-ras/JNK/c-Jun/cyclin D1信号通路的研究[J].生物化学与生物物理进展,2008,35(5):570-576. 被引量:10
  • 7Tournier C, Hess P, Yang DD, et al. Requirement of JNK for stress - induced activation of the cytochrome c - mediated death pathway [ J ]. Science, 2000,288 (5467) : 870-874.
  • 8郑江华,陈开,陈祖林.JNK信号通路在δ氨基酮戊酸-光动力疗法诱导SW480细胞凋亡中的作用机制[J].世界华人消化杂志,2008,16(33):3792-3795. 被引量:2
  • 9Xu YM, Zhu F, Cho YY, et al. Extracellular signal - regulated kinase 8 - mediated c - Jun phosphorylation increases tumori- genesis of human colon cancer[ J ]. Cancer Res,2010, 70 (8) : 3218-3227.
  • 10Ogunwobi OO, Beales IL. The anti - apoptotic and growth stimu- latory actions of leptin in human colon cancer cells involves acti- vation of JNK mitogen activated protein kinase, JAK2 and PI3 kinase/Akt [J]. Int J Colorectal Dis ,2007,22 ( 4 ) :401-409.

二级参考文献43

  • 1郑江华,时德,陈祖林.δ氨基酮戊酸光动力疗法对人结肠癌细胞SW480游离钙浓度的影响[J].中华实验外科杂志,2005,22(2):250-250. 被引量:6
  • 2王晓艳,沈守荣,刘芬,李晓玲,范松青.NGX6基因对人结肠癌细胞HT-29细胞周期的影响[J].生物化学与生物物理进展,2006,33(1):45-50. 被引量:14
  • 3栾巍,黄海辉,梁后杰.SAPK/JNK信号通路在鼻咽癌放疗群集耐受中的作用研究[J].第三军医大学学报,2006,28(21):2177-2179. 被引量:3
  • 4周燕红,于皆平,罗和生,黄永兰,明媚,于红刚.胃泌素促FAK-Src-ERK1/2通路的活化对大肠癌细胞运动和侵袭的影响[J].中华内科杂志,2007,46(4):318-319. 被引量:4
  • 5Johansen LS. [Photodynamic therapy. A new method for the treatment of cancer] Ugeskr Laeger 1999; 161:3992-3995
  • 6Assefa Z, Vantieghem A, Declercq W, Vandenabeele P, Vandenheede JR, Merlevede W, de Witte P, Agostinis P. The activation of the c-Jun N-terminal kinase and p38 mitogen-activated protein kinase signaling pathways protects HeLa cells from apoptosis following photodynamic therapy with hypericin. J Biol Chem 1999; 274:8788-8796
  • 7Xue L, He J, Oleinick NL. Promotion of photodynamic therapy-induced apoptosis by stress kinases. Cell Death Differ 1999; 6:855-864
  • 8Chert YR, Meyer CF, Tan TH. Persistent activation of c-Jun N-terminal kinase 1 (JNK1) in gamma radiation-induced apoptosis. J Biol Chem 1996; 271: 631-634
  • 9Kick G, Messer G, Plewig G, Kind P, Goetz AE. Strong and prolonged induction of c-jun and c-fos proto-oncogenes by photodynamic therapy. Br J Cancer 1996; 74:30-36
  • 10Klotz LO, Fritsch C, Briviba K, Tsacmacidis N, Schliess F, Sies H. Activation of JNK and p38 but not ERK MAP kinases in human skin cells by 5-aminolevulinate-photodynamic therapy. Cancer Res 1998; 58:4297-4300

共引文献19

同被引文献18

  • 1杨昌卫.扶正抗癌方联合吉非替尼治疗晚期非小细胞肺癌的临床研究[D].广州:广州中医药大学,2011.
  • 2杨小兵,吴万垠,龙顺钦,等.扶正抗癌方联合吉非替尼对H1650裸鼠移植瘤EGFR、AKT及ERK表达的影响[C].广州:第五届国际中医、中西医结合肿瘤学术交流大会暨第十四届全国中西医结合肿瘤学术大会,2014.
  • 3杨小兵,吴万垠,龙顺钦,等.扶正抗癌方联合吉非替尼对肺癌H1650细胞株裸鼠移植瘤的抑制作用[C].广州:第五届国际中医、中西医结合肿瘤学术交流大会暨第十四届全国中西医结合肿瘤学术大会,2014.
  • 4杨小兵,陈晓,吴万垠,等.扶正抗癌方联合吉非替尼对肺癌A549细胞的影响及机制研究[c].重庆:第一届青年中西医结合肿瘤学术论坛,2015.
  • 5Yang XB,Wu WY,Long SQ,et al.Effect of gefitinib plus Chi- nese herbal medicine ( CHM) in patients with advanced non - small - cell lung cancer:a retrospective case - control study [J].Complement Ther Med,2014,22(6):1010-1018.
  • 6Toumier C.The 2 Faces of JNK Signaling in Cancer[J].Genes Cancer,2013,4(9/10):397-400.
  • 7Qing - Bai S,Nanyue C,Bode AM,et al.Deficiency of c - Jun - NH(2)- terminal kinase - 1 in mice enhances skin tumor develop- ment by 12-0- tetradecanoylphorbol -13- acetate[J].Cancer Res,2002,62(5):1343-1348.
  • 8Winn RA,Marek L,Han SY,et al.Restoration of Wnt -7a ex- pression reverses non - small cell lung cancer cellular transformation through frizzled -9- mediated growth inhibition and promotion of cell differentiation[J].J Biol Chem,2005,280(20):19625-19634.
  • 9Wu J,Zhao S,Tang Q,et al.Activation of SAPK/JNK mediated the inhibition and reciprocal interaction of DNA methyltransferase I and EZH2 by ursolic acid in human lung cancer cells [J].J Exp Clin Cancer Res,2015,34:99.
  • 10Kwon Y W,Jang S,Paek JS,et al.E- Ras improves the effi- ciency of reprogramming by facilitating cell cycle progression through JNK - Spl pathway[J].Stem Cell Res,2015,15(3):481-494.

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