摘要
RNA干扰(RNAi)为基因治疗提供了一个全新的思路。但单一的RNAi治疗只针对单个基因,因而最终无法治愈多基因变异引起的肿瘤。为了克服RNAi单基因治疗的不足,研究人员提出了RNA组合干扰(combinatorial RNA interference,coRNAi)的治疗策略。coRNAi通过联合应用针对单个或多个靶基因的RNAi诱发物以及其他RNA或蛋白沉默因子实现沉默靶基因、抑制肿瘤生长和促进肿瘤细胞凋亡。本文重点介绍了coRNAi的主要联合方式以及在肿瘤基因治疗中的研究进展。
RNA interference（RNAi） has been proved as a novel approach for gene therapy.However,RNAi mono-therapy only aims at single gene,it therefore may ultimately fail to cure cancers caused by polygene variation.To overcome the deficiency of RNAi mono-therapy,＂combinatorial RNA interference＂（coRNAi） was put forward as a new strategy.By co-expressing the inducers of RNAi triggering single or multiple targets directly and other RNA-or protein-based silencers,coRNAi keeps target genes silent,prevents carcinogenic progression and induces apoptosis of tumor cells.This paper mainly reviews the major strategies of coRNAi and their applications in cancer gene therapy.
出处
《药学学报》
CAS
CSCD
北大核心
2012年第5期573-579,共7页
Acta Pharmaceutica Sinica
基金
国家自然科学基金资助项目(81072578
30900822)
福建省发改委项目(2009.2.5)
教育部科学技术研究重点项目(210252)
