摘要
目的研究白花蛇舌草总黄酮的超声提取工艺。方法以超声波法提取总黄酮类物质,采用正交实验优化提取工艺。利用聚酰胺柱色谱法纯化提取的总黄酮,以芦丁为对照品,利用NaNO2-Al(NO3)3-NaOH显色法计算总黄酮含量。结果最佳提取工艺为白花蛇舌草药材以70%乙醇为提取溶剂,料液比1∶20,超声提取30 min,提取3次,提取率2.38%。结论优选出的最佳工艺简单易行,能完全提取白花蛇舌草中总黄酮。
Objective To study the ultrasonic extraction technique for the total favonoids from Hedyotis diffusa Willd.Methods To extract the total favonoids with an ultrasonic method,optimize the extraction process with the orthogonal experiment and purify them by chromatography with polyamide（PA） column using rutin as standard.The content of total favonoids was calculated by NaNO2-Al（NO3）3-NaOH.chromogenic method.Results The optimized extraction technique was that extracting Hedyotis diffusa Willd 30 min once for three times with 70% ethanol（raw material：solvent=1：20） as solvent,and the yield of which reached 2.38%.Conclusion The best extraction technique is easy and practical,which assuring the complete extraction of the total favonoids from Hedyotis diffusa Willd.
出处
《医药导报》
CAS
北大核心
2012年第5期640-642,共3页
Herald of Medicine
关键词
白花蛇舌草
总黄酮
正交实验
超声波提取
紫外分光光度法
Hedyotis diffusa Willd
Total flavonoids
Orthogonal experiment
Ultrasonic extraction
Ultraviolet spectrophotometry
