摘要
目的探讨脂联素(adiponectin,APN)在代谢综合征(metabolic syndrome,MS)发病机制中的可能作用及其与MS各组分的关系。方法 90只大鼠随机分成3组:MS正常盐模型组(NS组)、MS高盐模型组(HS组)、正常对照组(NC组),NS组大鼠给予高脂高糖饲料喂养,HS组大鼠给予高脂高糖高盐饲料喂养,NC组大鼠以基础饲料喂养。饲养过程中,每隔2w测量各组大鼠的体长、体质量、血脂、收缩压(SBP)、舒张压(DBP)、空腹血糖(FPG)等指标。通过2种不同的饲料联合链脲佐菌素(STZ)诱导,成功建立代谢综合征大鼠模型后,MS组(NS组和HS组)和NC组大鼠运用逆转录-聚合酶链反应(RT-PCR)技术,检测网膜脂肪组织APN mRNA的表达水平。结果 MS组大鼠网膜脂肪组织APN mRNA的表达水平低于NC组大鼠,差异具有统计学意义(P<0.05)。在MS组大鼠中,NS组大鼠网膜脂肪组织APN mRNA的表达水平与HS组比较,差异无统计学意义(P>0.05)。相关分析显示:网膜脂肪组织APN mRNA的表达水平与FPG呈负相关(r=-0.334,P<0.05),与体长呈负相关(r=-0.387,P<0.05),与体质量呈负相关(r=-0.327,P<0.05)。结论 MS大鼠网膜脂肪组织APN mR-NA的表达水平可能与MS的发病呈负相关。FPG、体质量、体长可能对MS大鼠网膜脂肪组织APN的mRNA表达水平存在负反馈作用。
Objective To establish the rat metabolic syndrome mode and research the adiponectin （APN） mRNA expression level in omental tissue. Analyze the APN effect and relate factor. Methods Ninty SDrats were randomly divided into 3 groups, normal-salt plus High-glucose fat diet group （NS）, high glu cose-fat-salt diet group （HS） and the normal control group （NC）. The rats in NS group were fed with nor mal salt plus high sucrose-fat diet for totally 12 weeks;the rats in HS group were fed with high sucrose-fat- salty diet for totally 12 weeks; the rats in NC group were fed with normal diet. In breeding process, every rat′s length, weight, lipids, systolic blood pressure （SBP）, diastolic blood pressure （DBP） and fasting plasma glucose （FPG） were all measured every 2 weeks. Through two kinds of diet joint induced with streptozocin （STZ） were established metabolic syndrome （MS） model of rats, mRNA expression levels of APN in omentum adipose tissues of rats were detected by reverse transcription polymerase chain reaction （RT-PCR）. Results APN mRNA expression level of omental adipose tissues was significantly lower than that of NC group （P d0.05）. The level of APN mRNA expression was not significant different in NS group than in HS group （P）0.05）. Correlation analysis showed that the level of APN mRNA expression in omental adipose tissues was negatively correlated with fasting glucose （r=-0. 334, P dO. 05）, weight （r=- 0. 327,P d0.05） and length （r=-0. 387,P d0.05）. Conclusion The level of APN mRNA ex pression in omental adipose tissues may be negative with metabolic syndrome. Fasting glucose, weight and length may has feedback action that affects APN mRNA expression in omental adipose tissues.
出处
《新疆医科大学学报》
CAS
2012年第5期575-579,共5页
Journal of Xinjiang Medical University
基金
国家自然科学基金项目(30960363)
新疆医科大学大学生创新性实验计划项目
关键词
代谢综合征大鼠模型
脂联素
逆转录-聚合酶链反应
metabolic syndrome rat mode
adiponectin
reverse transcription polymerase chain reaction（RT-PCR） ~
